QQ客服 官方微博 反馈留言

	Silver nanoparticles impede phorbol myristate acetate-induced monocyte-macrophage differentiation and autophagy
	Xu, Yingying; Wang, Liming; Bai, Ru; Zhang, Tianlu; Chen, Chunying
刊名	Nanoscale
	2015
卷号	7期号:38页码:16100-16109
ISSN号	2040-3364
DOI	10.1039/c5nr04200c
通讯作者	Chen, chunying(chenchy@nanoctr.cn)
英文摘要	Monocytes/macrophages are important constituents of the innate immune system. monocyte-macrophage differentiation is not only crucial for innate immune responses, but is also related to some cardiovascular diseases. silver nanoparticles (agnps) are one of the most widely used nanomaterials because of their broad-spectrum antimicrobial properties. however, the effect of agnps on the functions of blood monocytes is scarcely reported. here, we report the impedance effect of agnps on thp-1 monocyte differentiation, and that this effect was mediated by autophagy blockade and lysosomal impairment. firstly, agnps inhibit phorbol 12-myristate 13-acetate (pma)-induced monocyte differentiation by down-regulating both expression of surface marker cd11b and response to lipopolysaccharide (lps) stimulation. secondly, autophagy is activated during pma-induced thp-1 monocyte differentiation, and the autophagy inhibitor chloroquine (cq) can inhibit this process. thirdly, agnps block the degradation of the autophagy substrate p62 and induce autophagosome accumulation, which demonstrates the blockade of autophagic flux. fourthly, lysosomal impairments including alkalization and decrease of lysosomal membrane stability were observed in agnp-treated thp-1 cells. in conclusion, we demonstrate that the impedance of monocyte-macrophage differentiation by agnps is mediated by autophagy blockade and lysosomal dysfunction. our results suggest that crosstalk exists in different biological effects induced by agnps.
WOS关键词	MAMMALIAN AUTOPHAGY ; CELL ; CYTOTOXICITY ; INDUCTION ; APOPTOSIS ; STRESS ; CHO-K1
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000361834100066
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176399
专题	高能物理研究所
通讯作者	Chen, Chunying
作者单位	Natl Ctr Nanosci & Technol China, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Xu, Yingying,Wang, Liming,Bai, Ru,et al. Silver nanoparticles impede phorbol myristate acetate-induced monocyte-macrophage differentiation and autophagy[J]. Nanoscale,2015,7(38):16100-16109.
APA	Xu, Yingying,Wang, Liming,Bai, Ru,Zhang, Tianlu,&Chen, Chunying.(2015).Silver nanoparticles impede phorbol myristate acetate-induced monocyte-macrophage differentiation and autophagy.Nanoscale,7(38),16100-16109.
MLA	Xu, Yingying,et al."Silver nanoparticles impede phorbol myristate acetate-induced monocyte-macrophage differentiation and autophagy".Nanoscale 7.38(2015):16100-16109.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。