Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians
	Zhu, L ; Song, LS ; Xu, W ; Qian, PY
刊名	FISH & SHELLFISH IMMUNOLOGY
	2008
卷号	25 期号:3 页码:231-238
关键词	C-type lectin CRD Argopecten irradians tissue distribution mRNA expression injury healing immune response
ISSN号	1050-4648
通讯作者	boqianpy@ust.hk
中文摘要	C-type lectins are Ca2+-dependent carbohydrate-recognition proteins that play crucial roles in innate immunity. The cDNA of C-type lectin (AiCTL1) in the bay scallop Argopecten irradians was cloned by expressed sequence tag (EST) and RACE techniques. The full-length cDNA of AiCTL1 was 660 bp, consisting of a T-terminal. untranslated region (UTR) of 30 bp and a 3' UTR of 132 bp with a polyadenylation signal sequence AATAAA and a poly(A) tail. The AiCTL1 cDNA encoded a polypeptide of 166 amino acids with a putative signal peptide of 20 amino acid residues and a mature protein of 146 amino acids. The deduced amino acid sequence of AiCTL1 was highly similar to those of the C-type lectins from other animals and contained a typical carbohydrate-recognition domain (CRD) of 121 residues, which has four conserved disulfide-bonded cysteine residues that define the CRD and two additional cysteine residues at the amino terminus. AiCTL1 mRNA was dominantly expressed in the hemocytes of the bay scallop. The temporal expression of AiCTL1 mRNA in hemocytes was increased by 5.7-and 4.9-fold at 6 h after injury and 8 h after injection of bacteria, respectively. The structural features, high similarity and expression pattern of AiCTL1 indicate that the gene may be involved in injury heating and the immune response in A. irradians. (c) 2008 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别	SCI
原文出处	SEP
WOS记录号	WOS:000259759700006
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5957]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Zhu, L,Song, LS,Xu, W,et al. Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians[J]. FISH & SHELLFISH IMMUNOLOGY,2008,25(3):231-238.
APA	Zhu, L,Song, LS,Xu, W,&Qian, PY.(2008).Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians.FISH & SHELLFISH IMMUNOLOGY,25(3),231-238.
MLA	Zhu, L,et al."Molecular cloning and immune responsive expression of a novel C-type lectin gene from bay scallop Argopecten irradians".FISH & SHELLFISH IMMUNOLOGY 25.3(2008):231-238.