Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths

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	Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths
	Li, Xiaoyan 1; Anken, Ralf H.2,3; Wang, Gaohong 1; Hilbig, Reinhard 3; Liu, Yongding 1
刊名	MICROGRAVITY SCIENCE AND TECHNOLOGY
	2011
卷号	23 期号:1 页码:13-18
关键词	Vestibular system Development Functional weightlessness
ISSN号	0938-0108
通讯作者	Liu, YD, Chinese Acad Sci, Inst Hydrobiol, Donghunanlu 7, Wuhan 430072, Hunan, Peoples R China ; ralf.anken@dlr.de, liuyd@ihb.ac.cn
中文摘要	Stimulus dependence is a general feature of developing sensory systems. It has been shown earlier that the growth of otoliths of late-stage Cichlid fish (Oreochromis mossambicus) and Zebrafish (Danio rerio) was slowed down by hypergravity, whereas microgravity during spaceflight yielded an opposite effect, i.e., larger than 1 g otoliths, in Swordtail (Xiphophorus helleri) late-stage embryos. Using ground-based techniques to apply simulated weightlessness, long-term clinorotation (exposure on a fast-rotating clinostat with one axis of rotation for 7 days) led to larger than 1 g otoliths in late-stage Cichlid fish, which is fully in line with the results obtained on Swordtails from spaceflight. Hitherto, early-staged fish have not yet been subjected to (simulated or real) long-term (i.e., more than 3 or 4 days) weightlessness to investigate otolith growth. The present study was carried out in order to fill this gap. Therefore, we subjected Zebrafish at a somite-stage to Wall Vessel Rotation (WVR; a method regarded to provide simulated weightlessness), when the anlage of the inner ear already is present (10 h post fertilisation, hpf). Siblings were maintained under WVR for 3, 6, 9 and 12 days. Further short-term experiments (3 days) were carried out on 10 hpf animals as well as on very early larvae (1 K cell stage, 3 hpf) at two different rotation speeds. WVR (both rotation speeds) had no effect on otolith biogenesis in both stages as all otoliths were present after the experiments. In comparison with 1 g controls, WVR had significantly increased otolith growth (normalised by fish length) after 3 and 6 days of exposure, but significant differences of otolith growth between experimental animals and controls were not found after 9 and 12 days. In conclusion, WVR (at least within a time-span of exposure of up to 6 days) brings, comparable to the situation in real microgravity, a kind of feedback mechanism into action, resulting in larger otoliths. Later, possible effects of WVR might be overruled by normal allometric growth since the action of the feedback mechanism may be discontinued in the course of an adaptation.
英文摘要	Stimulus dependence is a general feature of developing sensory systems. It has been shown earlier that the growth of otoliths of late-stage Cichlid fish (Oreochromis mossambicus) and Zebrafish (Danio rerio) was slowed down by hypergravity, whereas microgravity during spaceflight yielded an opposite effect, i.e., larger than 1 g otoliths, in Swordtail (Xiphophorus helleri) late-stage embryos. Using ground-based techniques to apply simulated weightlessness, long-term clinorotation (exposure on a fast-rotating clinostat with one axis of rotation for 7 days) led to larger than 1 g otoliths in late-stage Cichlid fish, which is fully in line with the results obtained on Swordtails from spaceflight. Hitherto, early-staged fish have not yet been subjected to (simulated or real) long-term (i.e., more than 3 or 4 days) weightlessness to investigate otolith growth. The present study was carried out in order to fill this gap. Therefore, we subjected Zebrafish at a somite-stage to Wall Vessel Rotation (WVR; a method regarded to provide simulated weightlessness), when the anlage of the inner ear already is present (10 h post fertilisation, hpf). Siblings were maintained under WVR for 3, 6, 9 and 12 days. Further short-term experiments (3 days) were carried out on 10 hpf animals as well as on very early larvae (1 K cell stage, 3 hpf) at two different rotation speeds. WVR (both rotation speeds) had no effect on otolith biogenesis in both stages as all otoliths were present after the experiments. In comparison with 1 g controls, WVR had significantly increased otolith growth (normalised by fish length) after 3 and 6 days of exposure, but significant differences of otolith growth between experimental animals and controls were not found after 9 and 12 days. In conclusion, WVR (at least within a time-span of exposure of up to 6 days) brings, comparable to the situation in real microgravity, a kind of feedback mechanism into action, resulting in larger otoliths. Later, possible effects of WVR might be overruled by normal allometric growth since the action of the feedback mechanism may be discontinued in the course of an adaptation.
学科主题	Engineering, Aerospace; Thermodynamics; Mechanics
WOS标题词	Science & Technology ; Technology ; Physical Sciences
类目[WOS]	Engineering, Aerospace ; Thermodynamics ; Mechanics
研究领域[WOS]	Engineering ; Thermodynamics ; Mechanics
关键词[WOS]	FISH OREOCHROMIS-MOSSAMBICUS ; VESTIBULOOCULAR REFLEX ; CICHLID FISH ; CRITICAL PERIOD ; HYPERGRAVITY ; MINERALIZATION ; ADAPTATION ; SYSTEM ; CELLS
收录类别	SCI
资助信息	National Natural Science foundation of China [30400093]
语种	英语
WOS记录号	WOS:000286396000002
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15510]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hunan, Peoples R China 2.German Aerosp Ctr, Inst Aerosp Med, D-51147 Cologne, Germany 3.Univ Hohenheim, Inst Zool, D-70593 Stuttgart, Germany
推荐引用方式 GB/T 7714	Li, Xiaoyan,Anken, Ralf H.,Wang, Gaohong,et al. Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths[J]. MICROGRAVITY SCIENCE AND TECHNOLOGY,2011,23(1):13-18.
APA	Li, Xiaoyan,Anken, Ralf H.,Wang, Gaohong,Hilbig, Reinhard,&Liu, Yongding.(2011).Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths.MICROGRAVITY SCIENCE AND TECHNOLOGY,23(1),13-18.
MLA	Li, Xiaoyan,et al."Effects of Wall Vessel Rotation on the Growth of Larval Zebrafish Inner Ear Otoliths".MICROGRAVITY SCIENCE AND TECHNOLOGY 23.1(2011):13-18.