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	Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species
	Rao, Yu1,2; Zhong, Liqiao1,2; Liao, Tao1; Jin, Shiwei1; Wang, Yuzhen1; Song, Bo1; Li, Jia1,2; Zhang, Xiaohua1; Hemmingsen, Sean M.3; Xu, Ying1
刊名	DISEASES OF AQUATIC ORGANISMS
	2010-12-07
卷号	93期号:1页码:83-91
关键词	Vitellogenin detection Cyprinid fish Phage display ScFv antibody Bioassay Binding affinity
ISSN号	0177-5103
通讯作者	Dai, HP, Chinese Acad Sci, Inst Hydrobiol, 7 So E Lake Rd, Wuhan 430072, Peoples R China ; hpdai@ihb.ac.cn
中文摘要	Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
英文摘要	Various polyclonal and monoclonal antibodies have been developed for vitellogenin (Vtg) bioassays in different aquatic species. Preparation of these reagents is time-consuming and expensive. In the present study, a phage-displayed, recombinant, single-chain variable fragment (scFv) format antibody library was constructed using splenic mRNA from non-immunized mice. After 3 rounds of panning, 3 scFv antibodies with specificity for the highly conserved N-terminal region of cyprinid fish Vtg were isolated. One of these, antibody H4, bound purified Vtg from common carp Cyprinus carpio, zebrafish Danio rerio and Chinese rare minnow Gobiocypris rarus with similar affinities and detected Vtg in zebrafish plasma samples. This study provides a simple, low cost Vtg bioassay for plasma samples from a variety of cyprinid fish.
学科主题	Fisheries; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Veterinary Sciences
关键词[WOS]	ZEBRAFISH DANIO-RERIO ; MINNOW PIMEPHALES-PROMELAS ; LINKED-IMMUNOSORBENT-ASSAY ; ESTROGENIC CHEMICALS ; GOBIOCYPRIS-RARUS ; CARP ; BIOMARKER ; IMMUNOASSAY ; ELISA ; PURIFICATION
收录类别	SCI
资助信息	National Natural Science Foundation of China [20777091]; Chinese 863 program [2006AA06Z424]
语种	英语
WOS记录号	WOS:000285305100008
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15530]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Natl Res Council Canada, Plant Biotechnol Inst, Saskatoon, SK S7N 0W9, Canada
推荐引用方式 GB/T 7714	Rao, Yu,Zhong, Liqiao,Liao, Tao,et al. Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species[J]. DISEASES OF AQUATIC ORGANISMS,2010,93(1):83-91.
APA	Rao, Yu.,Zhong, Liqiao.,Liao, Tao.,Jin, Shiwei.,Wang, Yuzhen.,...&Dai, Heping.(2010).Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species.DISEASES OF AQUATIC ORGANISMS,93(1),83-91.
MLA	Rao, Yu,et al."Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species".DISEASES OF AQUATIC ORGANISMS 93.1(2010):83-91.

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