Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation

doi:10.1007/s00425-018-2934-6

CORC > 武汉植物园 > 中国科学院武汉植物园

	Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation
	Wang, Zhouya 1; Yang, Xianpeng 1; Feng, Tao 1; Li, Juanjuan 1; Huang, Longyu 1; Yang, Baiming 2; Zhao, Huayan 3; Jenks, Matthew A.4; Yang, Pingfang 1,5; Lu, Shiyou 1,5
刊名	PLANTA
	2018-09-01
卷号	248 期号:3 页码:715-727
关键词	Arabidopsis thaliana CER2-LIKE proteins Cuticular wax Evolution Fatty acid elongase Localization Nelumbo nucifera NnCER2 Phylogeny
ISSN号	0032-0935
DOI	10.1007/s00425-018-2934-6
英文摘要	Identification of NnCER2 and NnCER2-LIKE from Nelumbo nucifera, which are required for the very-long-chain fatty acid elongation, provides new evidence that CER2 proteins are evolutionarily conserved across the eudicots. CER2-LIKE family proteins have been described as core components of the fatty acid elongase complex in Arabidopsis, maize, and rice, having specific function in synthesis of the C-30 to C-34 fatty acyl-CoA precursors of cuticular waxes. Little is known about the functional conservation in this gene family across species. In this study, two CER2-LIKE family proteins, NnCER2 and NnCER2-LIKE, were characterized from sacred lotus (Nelumbo nucifera), which is an ancient basal eudicot. The transcriptional expression of NnCER2 and NnCER2-LIKE was found in floating leaf blades, emergent petioles and vertical leaves, petals, and anthers. The NnCER2 and NnCER2-LIKE proteins were localized to the endoplasmic reticulum and nucleus. Overexpressing NnCER2 and NnCER2-LIKE in Arabidopsis led to alteration of cuticle wax structure in inflorescence stems, and this was associated with elevated 30, 32, and 34 carbon length wax compounds, and their derivatives. The different substrate specificities of NnCER2 and NnCER2-LIKE were explored using co-expression with AtCER6 in yeast cells. These findings provide clear evidence that the function of CER2 family proteins in producing VLCFAs is highly conserved across the eudicots.
资助项目	National Natural Science Foundation of China[31570186] ; National Natural Science Foundation of China[31770377]
WOS研究方向	Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000441357700016
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5523]
专题	中国科学院武汉植物园
通讯作者	Yang, Pingfang; Lu, Shiyou
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 2.Changchun Guoxin Modern Agr Sci & Technol Dev Co, Changchun 130061, Jilin, Peoples R China 3.Wuhan Inst Bioengn, Appl Biotechnol Ctr, Wuhan 430415, Hubei, Peoples R China 4.West Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26505 USA 5.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Wang, Zhouya,Yang, Xianpeng,Feng, Tao,et al. Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation[J]. PLANTA,2018,248(3):715-727.
APA	Wang, Zhouya.,Yang, Xianpeng.,Feng, Tao.,Li, Juanjuan.,Huang, Longyu.,...&Lu, Shiyou.(2018).Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation.PLANTA,248(3),715-727.
MLA	Wang, Zhouya,et al."Evolutionarily conserved function of the sacred lotus (Nelumbo nucifera Gaertn.) CER2-LIKE family in very-long-chain fatty acid elongation".PLANTA 248.3(2018):715-727.