A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila

doi:10.1080/15384101.2018.1431595

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	A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila
	Zhang, Jing 1,2; Ma, Yang 1,2; Xiong, Jie 1; Miao, Wei 1; Yan, Guanxiong 1,2; Tian, Miao 1
刊名	CELL CYCLE
	2018
卷号	17 期号:5 页码:634-642
关键词	Tetrahymena thermophila meiosis transcription factor E2fl1/Dpl2 complex network
ISSN号	1538-4101
DOI	10.1080/15384101.2018.1431595
英文摘要	Evolutionarily conserved E2F family transcription factors regulate the cell cycle via controlling gene expression in a wide range of eukaryotes. We previously demonstrated that the meiosis-specific transcription factor E2fl1 had an important role in meiosis in the model ciliate Tetrahymena thermophila. Here, we report that expression of another E2F family transcription factor gene DPL2 correlates highly with that of E2FL1. Similar to e2fl1 Delta cells, dpl2 Delta cells undergo meiotic arrest prior to anaphase I, with the five chromosomes adopting an abnormal tandem arrangement. Immunofluorescence staining and immunoprecipitation experiments demonstrate that Dpl2 and E2fl1 form a complex during meiosis. We previously identified several meiotic regulatory proteins in T. thermophila. Cyc2 and Tcdk3 may cooperate to initiate meiosis and Cyc17 is essential for initiating meiotic anaphase. We investigate the relationship of these regulators with Dpl2 and E2fl1, and then construct a meiotic regulatory network by measuring changes in meiotic genes expression in knockout cells. We conclude that the E2fl1/Dpl2 complex plays a central role in meiosis in T. thermophila.
资助项目	Natural Science Foundation of China[31525021] ; Natural Science Foundation of China[91631303] ; Projects of International Cooperation and Exchanges Ministry of Science and Technology of China[2013DFG32390]
WOS研究方向	Cell Biology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000430934900013
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5166]
专题	中国科学院武汉植物园
通讯作者	Miao, Wei
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jing,Ma, Yang,Xiong, Jie,et al. A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila[J]. CELL CYCLE,2018,17(5):634-642.
APA	Zhang, Jing,Ma, Yang,Xiong, Jie,Miao, Wei,Yan, Guanxiong,&Tian, Miao.(2018).A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila.CELL CYCLE,17(5),634-642.
MLA	Zhang, Jing,et al."A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila".CELL CYCLE 17.5(2018):634-642.