Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System

doi:10.1021/acs.analchem.7b03584

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	Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System
	Li, Wei 2; Zhang, Zhiping 2; Zhang, Xiaowei 2; Wu, Guoqiang 2; Cui, Zongqiang 2; Tan, Tianwei 3; Zhang, Xian-En 1; Ma, Yingxin 2,3; Wang, Mingxiu 2
刊名	ANALYTICAL CHEMISTRY
	2017-12-05
卷号	89 期号:23 页码:12896-12901
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b03584
英文摘要	HIV latency is one of the major problems in HIV/AIDS cure. Imaging single-copy integrated proviral HIV DNA in host cell has both virology and clinical significance but remains technical challenge. Here, we developed a dual-color labeled CRISPR system to image the HIV-1 integrated proviral DNA in latently infected cells. The pair of CRISPRs was fluorescently labeled with two different color QDs using two alternative bioorthogonal ligation reactions. Integrated HIV-sequences are successfully mapped based on the colocalized signals of QDs in living cells. Compared to the existing zinc finger proteins and TALENs, the CRISPR system is much easier to operate and more efficient in imaging of internal genomic loci. Therefore, the proposed method proviral HIV-1, but also a versatile platform to image single genomic loci in living could be not cells. only a powerful tool for imaging
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000417549600041
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/4452]
专题	中国科学院武汉植物园
通讯作者	Cui, Zongqiang; Zhang, Xian-En
作者单位	1.Chinese Acad Sci, Inst Biophys, Ctr Biol Macromol, Natl Key Lab Biomacromol, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing Key Lab Bioproc, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Wei,Zhang, Zhiping,Zhang, Xiaowei,et al. Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System[J]. ANALYTICAL CHEMISTRY,2017,89(23):12896-12901.
APA	Li, Wei.,Zhang, Zhiping.,Zhang, Xiaowei.,Wu, Guoqiang.,Cui, Zongqiang.,...&Wang, Mingxiu.(2017).Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System.ANALYTICAL CHEMISTRY,89(23),12896-12901.
MLA	Li, Wei,et al."Live Visualization of HIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR System".ANALYTICAL CHEMISTRY 89.23(2017):12896-12901.