Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes

doi:10.1007/s00604-017-2300-6

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	Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes
	Cui, Zongqiang 2; Fayyadh, Thaer Kadhim 1,2,3; Ma, Fuying 1; Qin, Chong 2; Zhang, Xiaowei 2; Li, Wei 2; Zhang, Xian-En 4; Zhang, Zhiping 2
刊名	MICROCHIMICA ACTA
	2017-08-01
卷号	184 期号:8 页码:2815-2824
关键词	Coinfection detection Multiple viruses SpA-QDs Influenza a virus Human adenovirus
ISSN号	0026-3672
DOI	10.1007/s00604-017-2300-6
英文摘要	The simultaneous detection and evaluation of the coinfection of a cell by multiple viruses or even multiple sub-types still is a difficult challenge. The authors introduce a method for simultaneous imaging, detection and quantitative evaluation of multiple viruses in single cells by using multi-color quantum dot (QD) probes and in a single staining cycle. Themulticolor QD probes were fabricated via interaction between QDs conjugated to Staph. aureus protein A (SpA-QDs) and virus-specific antibodies. A cocktail of differently colored QD-SpA-MAbs probes were loaded into the same cells containing multiple viruses, and this enabled the different viruses to be fluorescently imaged and analyzed simultaneously. Specifically, influenza A viruses of type H1N1, H3N2, and H9N2, as well as human adenovirus species B type 3 (HAdV-B3) were imaged and detected in virus-infected cells or in their co-infected cells. In our perception, the method provides a flexible platform for simultaneous detection of multiple viruses in co-infected cells. Hence, it offers new opportunities for the molecular diagnosis of virus coinfection and for studies on virus-cell interactions.
资助项目	National Natural Science Foundation of China (NSFC)[31470269] ; Youth Innovation Promotion Association CAS ; Key Program of CAS ; Chinese Academy of Sciences[KJZD-EW-TZ-L04]
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000406066500037
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/4309]
专题	中国科学院武汉植物园
通讯作者	Cui, Zongqiang
作者单位	1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan, Peoples R China 2.Chinese Acad Sci, State Key Lab Virol, Wuhan Inst Virol, Wuhan, Peoples R China 3.MOH, Baghdad, Iraq 4.Chinese Acad Sci, Natl Lab Biomacromol, CAS Ctr Excellence Biomacromol, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cui, Zongqiang,Fayyadh, Thaer Kadhim,Ma, Fuying,et al. Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes[J]. MICROCHIMICA ACTA,2017,184(8):2815-2824.
APA	Cui, Zongqiang.,Fayyadh, Thaer Kadhim.,Ma, Fuying.,Qin, Chong.,Zhang, Xiaowei.,...&Zhang, Zhiping.(2017).Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes.MICROCHIMICA ACTA,184(8),2815-2824.
MLA	Cui, Zongqiang,et al."Simultaneous detection of multiple viruses in their co-infected cells using multicolour imaging with self-assembled quantum dot probes".MICROCHIMICA ACTA 184.8(2017):2815-2824.