Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits

doi:10.1186/s12870-014-0269-4

CORC > 武汉植物园 > 中国科学院武汉植物园

	Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits
	Liu,Yongliang 2,5; Zeng,Shaohua 3; Sun,Wei 1; Wu,Min 3; Hu,Weiming 2,5; Shen,Xiaofei 2,5; Wang,Ying 2,3,4
刊名	BMC Plant Biology
	2014-12-16
卷号	14 期号:1
关键词	Carotenoids Chromoplast Fruit development Gene expression Lycium barbarum L. ruthenicum
ISSN号	1471-2229
DOI	10.1186/s12870-014-0269-4
英文摘要	AbstractBackgroundThe traditional Chinese medicinal plants Lycium barbarum L. and L. ruthenicum Murr. are valued for the abundance of bioactive carotenoids and anthocyanins in their fruits, respectively. However, the cellular and molecular mechanisms contributing to their species-specific bioactive profiles remain poorly understood.ResultsIn this study, the red fruit (RF) of L. barbarum was found to accumulate high levels of carotenoids (primarily zeaxanthin), while they were undetectable in the black fruit (BF) of L. ruthenicum. Cytological and gene transcriptional analyses revealed that the chromoplast differentiation that occurs in the chloroplast during fruit ripening only occurs in RF, indicating that the lack of chromoplast biogenesis in BF leads to no sink for carotenoid storage and the failure to synthesize carotenoids. Similar enzyme activities of phytoene synthase 1 (PSY1), chromoplast-specific lycopene β-cyclase (CYC-B) and β-carotene hydroxylase 2 (CRTR-B2) were observed in both L. ruthenicum and L. barbarum, suggesting that the undetectable carotenoid levels in BF were not due to the inactivation of carotenoid biosynthetic enzymes. The transcript levels of the carotenoid biosynthetic genes, particularly PSY1, phytoene desaturase (PDS), β-carotene desaturase (ZDS), CYC-B and CRTR-B2, were greatly increased during RF ripening, indicating increased zeaxanthin biosynthesis. Additionally, carotenoid cleavage dioxygenase 4 (CCD4) was expressed at much higher levels in BF than in RF, suggesting continuous carotenoid degradation in BF.ConclusionsThe failure of the chromoplast development in BF causes low carotenoid biosynthesis levels and continuous carotenoid degradation, which ultimately leads to undetectable carotenoid levels in ripe BF. In contrast, the successful chromoplast biogenesis in RF furnishes the sink necessary for carotenoid storage. Based on this observation, the abundant zeaxanthin accumulation in RF is primarily determined via both the large carotenoid biosynthesis levels and the lack of carotenoid degradation, which are regulated at the transcriptional level.
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12870-014-0269-4
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/3508]
专题	中国科学院武汉植物园
通讯作者	Wang,Ying
作者单位	1.China Academy of Chinese Medical Sciences; Institute of Chinese Materia Medica 2.Chinese Academy of Sciences; Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden 3.Chinese Academy of Sciences; Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden 4.Chinese Academy of Sciences; Northwest Center for Agrobiotechnology (Ningxia) 5.University of the Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Liu,Yongliang,Zeng,Shaohua,Sun,Wei,et al. Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits[J]. BMC Plant Biology,2014,14(1).
APA	Liu,Yongliang.,Zeng,Shaohua.,Sun,Wei.,Wu,Min.,Hu,Weiming.,...&Wang,Ying.(2014).Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits.BMC Plant Biology,14(1).
MLA	Liu,Yongliang,et al."Comparative analysis of carotenoid accumulation in two goji (Lycium barbarum L. and L. ruthenicumMurr.) fruits".BMC Plant Biology 14.1(2014).