Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor

doi:10.3389/fmicb.2018.00791

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	Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor
	Zhang, Ju-Yuan 1; Lin, Gui-Ming 1; Xing, Wei-Yue 1,2; Zhang, Cheng-Cai 1
刊名	FRONTIERS IN MICROBIOLOGY
	2018-04-24
卷号	9 页码:10
关键词	cyanobacteria peptidoglycan growth pattern cell wall heterocyst HADA
ISSN号	1664-302X
DOI	10.3389/fmicb.2018.00791
英文摘要	Cyanobacteria were the first oxygenic photosynthetic organisms during evolution and were ancestors of plastids. Cyanobacterial cells exhibit an extraordinary diversity in their size and shape, and bacterial cell morphology largely depends on the synthesis and the dynamics of the peptidoglycan (PG) layer. Here, we used a fluorescence analog of the PG synthesis precursor D-Ala, 7-Hydroxycoumarin-amino-D-alanine (HADA), to probe the PG synthesis pattern in live cells of cyanobacteria with different morphology. They displayed diverse synthesis patterns, with some strains showing an intensive HADA incorporation at the septal region, whereas others gave an HADA signal distributed around the cells. Growth zones covering several cells at the tips of the filament were present in some filamentous strains such as in Arthrospira. In Anabaena PCC 7120, which is capable of differentiating heterocysts for N-2 fixation, PG synthesis followed the cell division cycle. In addition, an HADA incorporation was strongly activated from 12 to 15 h following the initiation of heterocyst development, indicating a thickening of the PG layer in heterocysts. The PG synthesis pattern is diverse in cyanobacteria and responds to developmental regulation. The use of fluorescent analogs may serve as a useful tool for understanding the mechanisms of cell growth and morphogenesis operating in these organisms.
资助项目	Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDJ-SSW-SMC016] ; Recruitment Program of Global Experts of China[Y523011]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000430711800001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/3395]
专题	中国科学院武汉植物园
通讯作者	Zhang, Cheng-Cai
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ju-Yuan,Lin, Gui-Ming,Xing, Wei-Yue,et al. Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor[J]. FRONTIERS IN MICROBIOLOGY,2018,9:10.
APA	Zhang, Ju-Yuan,Lin, Gui-Ming,Xing, Wei-Yue,&Zhang, Cheng-Cai.(2018).Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor.FRONTIERS IN MICROBIOLOGY,9,10.
MLA	Zhang, Ju-Yuan,et al."Diversity of Growth Patterns Probed in Live Cyanobacterial Cells Using a Fluorescent Analog of a Peptidoglycan Precursor".FRONTIERS IN MICROBIOLOGY 9(2018):10.