Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)

doi:10.3389/fpls.2017.01383

CORC > 武汉植物园 > 中国科学院武汉植物园

	Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)
	Yao, Xiaohong 2; Guo, Rui 2,3; Landis, Jacob B.1; Moore, Michael J.4; Meng, Aiping 2; Jian, Shuguang 5; Wang, Hengchang 2
刊名	FRONTIERS IN PLANT SCIENCE
	2017-08-25
卷号	8 页码:13
关键词	Actinidia eriantha high-throughput sequencing transcriptome EST-SSRs population genetic structure
ISSN号	1664-462X
DOI	10.3389/fpls.2017.01383
英文摘要	Actinidia eriantha Benth. is a diploid perennial woody vine native to China and is recognized as a valuable species for commercial kiwifruit improvement with high levels of ascorbic acid as well as having been used in traditional Chinese medicine. Due to the lack of genomic resources for the species, microsatellite markers for population genetics studies are scarce. In this study, RNASeq was conducted on fruit tissue of A. eriantha, yielding 5,678,129 reads with a total output of 3.41 Gb. De novo assembly yielded 69,783 non-redundant unigenes (41.3 Mb), of which 21,730 were annotated using protein databases. A total of 8,658 EST-SSR loci were identified in 7,495 unigene sequences, for which primer pairs were successfully designed for 3,842 loci (44.4%). Among these, 183 primer pairs were assayed for PCR amplification, yielding 69 with detectable polymorphism in A. eriantha. Additionally, 61 of the 69 polymorphic loci could be successfully amplified in at least one other Actinidia species. Of these, 14 polymorphic loci (mean N-A = 6.07 +/- 2.30) were randomly selected for assessing levels of genetic diversity and population structure within A. eriantha. Finally, a neighbor-joining tree and Bayesian clustering analysis showed distinct clustering into two groups (K = 2), agreeing with the geographical distributions of these populations. Overall, our results will facilitate further studies of genetic diversity within A. eriantha and will aid in discriminating outlier loci involved in local adaptation.
资助项目	Backbone Talent Program of Wuhan Botanical Garden, Chinese Academy of Sciences[Y655291A04] ; NSFC[31370223] ; NSTIPC[2013FY111200] ; SPRPCAS[XDA13020500] ; Natural Scientific Foundation of China[31770374]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000408377700001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1324]
专题	中国科学院武汉植物园
通讯作者	Yao, Xiaohong; Wang, Hengchang
作者单位	1.Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Oberlin Coll, Dept Biol, Oberlin, OH 44074 USA 5.Chinese Acad Sci, South China Bot Garden, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Yao, Xiaohong,Guo, Rui,Landis, Jacob B.,et al. Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)[J]. FRONTIERS IN PLANT SCIENCE,2017,8:13.
APA	Yao, Xiaohong.,Guo, Rui.,Landis, Jacob B..,Moore, Michael J..,Meng, Aiping.,...&Wang, Hengchang.(2017).Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae).FRONTIERS IN PLANT SCIENCE,8,13.
MLA	Yao, Xiaohong,et al."Development and Application of Transcriptome-Derived Microsatellites in Actinidia eriantha (Actinidiaceae)".FRONTIERS IN PLANT SCIENCE 8(2017):13.