Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop

doi:10.1111/j.1744-7909.2010.01026.x

CORC > 武汉植物园 > 中国科学院武汉植物园

	Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop
	Zhao, Hua 1; Yu, Jiangyan 1; You, Frank M.2; Luo, Mingcheng 2; Peng, Junhua 1,3
刊名	JOURNAL OF INTEGRATIVE PLANT BIOLOGY
	2011-03-01
卷号	53 期号:3 页码:232-245
ISSN号	1672-9072
DOI	10.1111/j.1744-7909.2010.01026.x
英文摘要	Miscanthus sinensis has high biomass yield and contributed two of the three genomes in M. x giganteus, a bioenergy crop widely studied in Europe and North America, and thus is a potential biomass crop and an important germplasm for Miscanthus breeding. Molecular markers are essential for germplasm evaluation, genetic analyses and new cultivar development in M. sinensis. In the present study, we reported transferability of simple sequence repeat (SSR) markers from Brachypodium distachyon to M. sinensis. A set of 57 SSR markers evenly distributed across the B. distachyon genome were deliberately designed. Out of these B. distachyon SSR markers, 86.0% are transferable to M. sinensis. The SSR loci amplified in M. sinensis were validated by re-sequencing the amplicons. The polymorphism information content (PIC) of the transferable SSR markers varied from 0.073 to 0.375 with a mean of 0.263, assessed based on 21 M. sinensis genotypes. Phylogenetic tree based on 162 alleles detected by 49 SSR markers could unambiguously distinguish B. distachyon from M. sinensis, and cluster 21 M. sinensis genotypes into three groups that are basically in coincidence with their geographical distribution and ecotype classifications. The markers developed by the comparative genomic approach could be useful for germplasm evaluation, genetic analysis, and marker-assisted breeding in Miscanthus.
资助项目	Chinese Academy of Sciences[KSCX2-YW-Z-0722] ; Chinese Academy of Sciences[KSCX2-YW-G-036] ; National Natural Science Foundation of China[30870233]
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000287589200004
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/890]
专题	中国科学院武汉植物园
通讯作者	Peng, Junhua
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA 3.Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA
推荐引用方式 GB/T 7714	Zhao, Hua,Yu, Jiangyan,You, Frank M.,et al. Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2011,53(3):232-245.
APA	Zhao, Hua,Yu, Jiangyan,You, Frank M.,Luo, Mingcheng,&Peng, Junhua.(2011).Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,53(3),232-245.
MLA	Zhao, Hua,et al."Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis, a Potential Biomass Crop".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 53.3(2011):232-245.