Chemical and Genetic Assessment of Variability in Commercial Radix Astragali (Astragalus spp.) by Ion Trap LC-MS and Nuclear Ribosomal DNA Barcoding Sequence Analyses

doi:10.1021/jf1028174

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	Chemical and Genetic Assessment of Variability in Commercial Radix Astragali (Astragalus spp.) by Ion Trap LC-MS and Nuclear Ribosomal DNA Barcoding Sequence Analyses
	Xiao, Wei-Lie 1,2,3; Motley, Timothy J.4; Unachukwu, Uchenna J.1,2; Lau, Clara Bik San 5; Jiang, Bei 6; Hong, Feng 3; Leung, Ping-Chung 5; Wang, Qing-Feng 4,7; Liyingston, Philip O.3; Cassileth, Barrie R.3
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2011-03-09
卷号	59 期号:5 页码:1548-1556
关键词	Radix Astragali Astragalus DNA barcoding LC-MS
ISSN号	0021-8561
DOI	10.1021/jf1028174
英文摘要	Radix Astragali (Huangqi) has been demonstrated to have a wide range of immunopotentiating effects and has been used as an adjuvant medicine during cancer therapy. Identity issues in the collection of Radix Astragali exist because many sympatric species of Astragalus occur in the northern regions of China. In order to assess the quality, purity, and uniformity of commercial Radix Astragali, 44 samples were purchased from herbal stores in Hong Kong and New York City. The main constituents, including four isoflavonoids and three saponins, were quantitatively determined by liquid chromatography mass spectrometry (LC-MS). There was significant sample-to-sample variability in the amounts of the saponins and isoflavonoids measured. Furthermore, DNA barcoding utilizing the variable nuclear ITS spacer regions of the 44 purchased Radix Astragali samples were sequenced, aligned and compared. Eight polymorphic point mutations were identified which separated the Radix Astragali samples into three groups. These results indicate that the chemical and genetic variability that exists among Radix Astragali medicinal products is still a consistency and quality issue for this herbal. Two-way ANOVA analysis showed significant effects on the contents of the seven tested compounds when both phylogenetic and geographic (i.e., point of purchase) factors were considered. Therefore, chemical profiles determined by LC-MS and DNA profiles in ITS spacer domains could serve as barcode markers for quality control of Radix Astragali.
资助项目	NIH[P50AT002779]
WOS研究方向	Agriculture ; Chemistry ; Food Science & Technology
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000287824400009
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/882]
专题	中国科学院武汉植物园
通讯作者	Kennelly, Edward J.
作者单位	1.CUNY, Lehman Coll, Bronx, NY USA 2.CUNY, Grad Ctr, Bronx, NY USA 3.Mem Sloan Kettering Canc Ctr, Integrat Med Serv, New York, NY 10021 USA 4.Old Dominion Univ, Dept Biol Sci, Norfolk, VA 23529 USA 5.Chinese Univ Hong Kong, Inst Chinese Med, Hong Kong, Hong Kong, Peoples R China 6.Dali Univ, Coll Pharm, Dali 671000, Yunnan, Peoples R China 7.Chinese Acad Sci, Wuhan Bot Inst, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Wei-Lie,Motley, Timothy J.,Unachukwu, Uchenna J.,et al. Chemical and Genetic Assessment of Variability in Commercial Radix Astragali (Astragalus spp.) by Ion Trap LC-MS and Nuclear Ribosomal DNA Barcoding Sequence Analyses[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2011,59(5):1548-1556.
APA	Xiao, Wei-Lie.,Motley, Timothy J..,Unachukwu, Uchenna J..,Lau, Clara Bik San.,Jiang, Bei.,...&Kennelly, Edward J..(2011).Chemical and Genetic Assessment of Variability in Commercial Radix Astragali (Astragalus spp.) by Ion Trap LC-MS and Nuclear Ribosomal DNA Barcoding Sequence Analyses.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,59(5),1548-1556.
MLA	Xiao, Wei-Lie,et al."Chemical and Genetic Assessment of Variability in Commercial Radix Astragali (Astragalus spp.) by Ion Trap LC-MS and Nuclear Ribosomal DNA Barcoding Sequence Analyses".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 59.5(2011):1548-1556.