Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana

doi:10.1093/jxb/erv477

CORC > 武汉植物园 > 中国科学院武汉植物园

	Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana
	Wang, Yanping 1; Yang, Li 1,2; Chen, Xi 3; Ye, Tiantian 1,2; Zhong, Bao 1,2; Liu, Ruijie 1,2; Wu, Yan 3; Chan, Zhulong 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2016
卷号	67 期号:1 页码:421-434
关键词	ABA signal reconstitution abscisic acid drought stress metabolite profile MLP43 reactive oxygen species SnRK2.6
ISSN号	0022-0957
DOI	10.1093/jxb/erv477
英文摘要	Drought stress is one of the disadvantageous environmental conditions for plant growth and reproduction. Given the importance of abscisic acid (ABA) to plant growth and abiotic stress responses, identification of novel components involved in ABA signalling transduction is critical. In this study, we screened numerous Arabidopsis thaliana mutants by seed germination assay and identified a mutant mlp43 (major latex protein-like 43) with decreased ABA sensitivity in seed germination. The mlp43 mutant was sensitive to drought stress while the MLP43-overexpressed transgenic plants were drought tolerant. The tissue-specific expression pattern analysis showed that MLP43 was predominantly expressed in cotyledons, primary roots and apical meristems, and a subcellular localization study indicated that MLP43 was localized in the nucleus and cytoplasm. Physiological and biochemical analyses indicated that MLP43 functioned as a positive regulator in ABA- and drought-stress responses in Arabidopsis through regulating water loss efficiency, electrolyte leakage, ROS levels, and as well as ABA-responsive gene expression. Moreover, metabolite profiling analysis indicated that MLP43 could modulate the production of primary metabolites under drought stress conditions. Reconstitution of ABA signalling components in Arabidopsis protoplasts indicated that MLP43 was involved in ABA signalling transduction and acted upstream of SnRK2s by directly interacting with SnRK2.6 and ABF1 in a yeast two-hybrid assay. Moreover, ABA and drought stress down-regulated MLP43 expression as a negative feedback loop regulation to the performance of MLP43 in ABA and drought stress responses. Therefore, this study provided new insights for interpretation of physiological and molecular mechanisms of Arabidopsis MLP43 mediating ABA signalling transduction and drought stress responses.
资助项目	National Natural Science Foundation of China[31370302] ; National Natural Science Foundation of China[31300233] ; 'Hundred Talents Program'[54Y154761O01076] ; 'Hundred Talents Program'[29Y329631O0263]
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000367816300031
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/156]
专题	中国科学院武汉植物园
通讯作者	Chan, Zhulong
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei Province, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanping,Yang, Li,Chen, Xi,et al. Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana[J]. JOURNAL OF EXPERIMENTAL BOTANY,2016,67(1):421-434.
APA	Wang, Yanping.,Yang, Li.,Chen, Xi.,Ye, Tiantian.,Zhong, Bao.,...&Chan, Zhulong.(2016).Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana.JOURNAL OF EXPERIMENTAL BOTANY,67(1),421-434.
MLA	Wang, Yanping,et al."Major latex protein-like protein 43 (MLP43) functions as a positive regulator during abscisic acid responses and confers drought tolerance in Arabidopsis thaliana".JOURNAL OF EXPERIMENTAL BOTANY 67.1(2016):421-434.