Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp WBG-1

doi:10.1186/s13068-016-0541-y

CORC > 武汉植物园 > 中国科学院武汉植物园

	Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp WBG-1
	Wen, Xiaobin 1; Du, Kui 1,2; Wang, Zhongjie 1; Peng, Xinan 1,2; Luo, Liming 1,3; Tao, Huanping 1; Xu, Yan 1,2; Zhang, Dan 1; Geng, Yahong 1; Li, Yeguang 1
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2016-06-13
卷号	9 页码:12
关键词	Graesiella Microalgae selection Lipid Mass culture Open raceway pond
ISSN号	1754-6834
DOI	10.1186/s13068-016-0541-y
英文摘要	Background: Commercial production of microalgal biodiesel is not yet economically viable, largely because of low storage lipid yield in microalgae mass cultivation. Selection of lipid-rich microalgae, thus, becomes one of the key research topics for microalgal biodiesel production. However, the laboratory screening protocols alone cannot predict the ability of the strains to dominate and perform in outdoor ponds. Comprehensive assessment of microalgae species should be performed not only under the laboratory conditions, but also in the fields. Results: Laboratory investigations using a bubbled column photobioreactor indicated the microalga Graesiella sp. WBG-1 to be the most productive species among the 63 Chlorophyta strains. In a 10 L reactor, mimicking the industrial circular pond, Graesiella sp. WBG-1 produced 12.03 g biomass m(-2) day(-1) and 5.44 g lipids (45.23 % DW) m-2 day-1 under 15 mol m-2 day-1 artificial light irradiations. The lipid content decreased to similar to 34 % DW when the microalga was cultured in 30 L tank PBR under natural solar irradiations, but the decline of lipid content with scaling up was the minimum among the tested strains. Based on these results, the microalga was further tested for its lipid production and culture competitiveness using a pilot-scale raceway pond (200 m(2) illuminated area, culture volume 40,000 L). Consequently, Graesiella sp. WBG-1 maintained a high lipid content (33.4 % DW), of which similar to 90 % was storage TAGs. Results from the outdoor experiments indicated the nice adaptability of the Graesiella sp. WBG-1 to strong and fluctuating natural solar irradiance and temperature, and also demonstrated several other features, such as large cell size (easy for harvest and resistant to swallow by protozoa) and tolerance to high culture pH (helpful to CO2 fixation). Conclusions: Graesiella sp. WBG-1 was a promising strain capable of accumulating large amount of storage lipid under nature solar irradiance and temperature. The high lipid content of 33.4 % DW was achieved for the first time in pilot-scale raceway pond. The results also provide evidence for the feasibility of using low-cost raceway pond for autotrophic cultivation of microalgae for biodiesel production.
资助项目	Ministry of Science and Technology of China[2012FY112900] ; Ministry of Science and Technology of China[2013AA065805] ; China Petrochemical Corporation[210080] ; China Petrochemical Corporation[215104]
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000378870100001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/60]
专题	中国科学院武汉植物园
通讯作者	Li, Yeguang
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Pant Germplasm Enhancement & Specialty Ag, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Baylor Coll Med, Dept Pathol & Immunol, Houston, TX 78703 USA
推荐引用方式 GB/T 7714	Wen, Xiaobin,Du, Kui,Wang, Zhongjie,et al. Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp WBG-1[J]. BIOTECHNOLOGY FOR BIOFUELS,2016,9:12.
APA	Wen, Xiaobin.,Du, Kui.,Wang, Zhongjie.,Peng, Xinan.,Luo, Liming.,...&Li, Yeguang.(2016).Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp WBG-1.BIOTECHNOLOGY FOR BIOFUELS,9,12.
MLA	Wen, Xiaobin,et al."Effective cultivation of microalgae for biofuel production: a pilot-scale evaluation of a novel oleaginous microalga Graesiella sp WBG-1".BIOTECHNOLOGY FOR BIOFUELS 9(2016):12.