Optimization and parameter study for chiral separation by capillary electrophoresis

	Optimization and parameter study for chiral separation by capillary electrophoresis
	Zhu, XF ; Lin, BC ; Jakob, A ; Epperlein, U ; Koppenhoefer, B
刊名	hrc-journal of high resolution chromatography
	1999-08-01
卷号	22 期号:8 页码:449-453
关键词	capillary electrophoresis experimental design cyclodextrin chiral separation
英文摘要	orthogonal design and uniform design were used for the optimization of separation of enantiomers using 2,6-di-o-methyl-beta-cyclodextrin (dm-beta-cd) as a chiral selector by capillary zone electrophoresis, the concentration of dm-beta-cd, buffer ph, running voltage, and capillary temperature were selected as variable parameters, their different effects on peak resolution were studied by the design methods. it was concluded that orthogonal design offers a rapid and efficient means for testing the importance of individual parameters and for determining the optimum operating conditions. however, for a large number of both factors and levels, uniform design is more efficient, the effect of addition of methanol and citric acid buffer on the separation of enantiomers was also examined.
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	micellar electrokinetic chromatography ; performance liquid-chromatography ; zone electrophoresis ; amino-acids ; cyclodextrins ; design ; phase ; enantiomers ; resolution ; drugs
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000083356000004
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/86221]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Beijing 100864, Peoples R China 2.Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany
推荐引用方式 GB/T 7714	Zhu, XF,Lin, BC,Jakob, A,et al. Optimization and parameter study for chiral separation by capillary electrophoresis[J]. hrc-journal of high resolution chromatography,1999,22(8):449-453.
APA	Zhu, XF,Lin, BC,Jakob, A,Epperlein, U,&Koppenhoefer, B.(1999).Optimization and parameter study for chiral separation by capillary electrophoresis.hrc-journal of high resolution chromatography,22(8),449-453.
MLA	Zhu, XF,et al."Optimization and parameter study for chiral separation by capillary electrophoresis".hrc-journal of high resolution chromatography 22.8(1999):449-453.