Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability | |
Liu DM(刘冬梅)1,2; Chen J(陈娟)1; Shi YP(师彦平)1; Chen J(陈娟); Shi YP(师彦平) | |
刊名 | Analytica Chimica Acta |
2018 | |
卷号 | 1006期号:0页码:90-98 |
关键词 | Tyrosinase Aminated Magnetic Nanoparticles Physical Adsorption Covalent Crosslinking Inhibitor Screening |
ISSN号 | 0003-2670 |
DOI | 10.1016/j.aca.2017.12.022 |
英文摘要 | In the present study, the immobilization method of physical adsorption combined with covalent crosslinking was developed to avoid the shortcomings of both the noncovalent and covalent coupling methods. For the first time, tyrosinase (TYR) was immobilized on the surface of aminated magnetic nanoparticles (Fe3O4-NH2) by the developed method. TYR was firstly adsorbed on the surface of Fe3O4-NH2 by electrostatic interaction, and then by covalent crosslinking with glutaraldehyde (GA), TYR was firmly immobilized on the supports. The immobilized TYR showed enhanced pH and temperature endurances at the optimum pH of 7.0 and temperature of 35 °C. TYR reusability was significantly improved after immobilization and it retained 61.4 ± 2.3% of its initial activity after 5 repeated cycles. Immobilized TYR also showed improved storage stability (73.2 ± 1.1% after 30 days of storage at 4 °C). In addition, the immobilized TYR showed a higher biological affinity to substrate owing to the stabilization of TYR in its active conformation by electrostatic interaction prior to covalent crosslinking. Finally, the immobilized TYR was used to screen of enzyme inhibitors from 11 traditional Chinese medicines (TCMs) to validate whether this method can be used for enzyme inhibitor screening or not. |
学科主题 | 分析化学与药物化学 |
资助项目 | 药物化学成分研究组 |
语种 | 英语 |
WOS记录号 | WOS:000425502600010 |
资助机构 | the National Natural Science Foundation of China (Nos. 21775153 ; 21575150) |
内容类型 | 期刊论文 |
源URL | [http://ir.licp.ac.cn/handle/362003/23738] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | Chen J(陈娟); Shi YP(师彦平) |
作者单位 | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, PR China |
推荐引用方式 GB/T 7714 | Liu DM,Chen J,Shi YP,et al. Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability[J]. Analytica Chimica Acta,2018,1006(0):90-98. |
APA | Liu DM,Chen J,Shi YP,Chen J,&Shi YP.(2018).Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability.Analytica Chimica Acta,1006(0),90-98. |
MLA | Liu DM,et al."Tyrosinase immobilization on aminated magnetic nanoparticles by physical adsorption combined with covalent crosslinking with improved catalytic activity, reusability and storage stability".Analytica Chimica Acta 1006.0(2018):90-98. |
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