Recovery of Protease Inhibitors from Potato Fruit Water by Expanded Bed Adsorption Chromatography in Pilot Scale | |
Jin CY(靳承煜)1,2; Zeng FK(曾凡逵)1![]() ![]() ![]() | |
刊名 | American Journal of Potato Research
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2018 | |
卷号 | 95期号:1页码:1-8 |
关键词 | Protease Inhibitors Expanded Bed Adsorption Potato Glycoalkaloids |
ISSN号 | 1099-209X |
DOI | 10.1007/s12230-017-9605-1 |
英文摘要 | The current study was conducted to investigate the recovery of native potato protein from potato fruit water (PFW) by expanded bed adsorption (EBA) chromatography. The eluted proteins were concentrated by ultrafiltration and spray-dried into powder. The SDS-PAGE showed that the recovered proteins were potato protease inhibitors (PPIs). The trypsin and chymotrypsin inhibitor activities of the recovered PPIs were 377.93 ± 8.22 and 12.90 ± 0.03 mg g−1 protein, respectively. The recovery yield of protease inhibitors was 74.88%. The glycoalkaloid assay showed that the recovered PPIs contained 30.31 ± 0.15 μg g−1 of α-chaconine and 92.77 ± 0.52 μg g−1 of α-solanine, and these values were much lower than those in potato protein concentrate (PPC) obtained by traditional thermal coagulation. The most abundant amino acid in the PPIs was serine. The results indicated that the EBA can be used to effectively recover native potato protein from PFW. |
学科主题 | 环境材料与生态化学 |
资助项目 | 材料与农业工程研究组 |
语种 | 英语 |
WOS记录号 | WOS:000422800500001 |
资助机构 | the West Light Foundation of The Chinese Academy of Sciences (The Science Development Talent Teach words [2013] No. 165) ; the Natural Science Foundation of Gansu Province (1501RJZA004) ; the National Natural Science Foundation of China (31301532) |
内容类型 | 期刊论文 |
源URL | [http://ir.licp.ac.cn/handle/362003/22828] ![]() |
专题 | 兰州化学物理研究所_环境材料与生态化学研究发展中心 |
通讯作者 | Ceng FK(曾凡逵) |
作者单位 | 1.Research & Development Center for Eco-material and Eco-Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, No.18, Tianshui Middle Road, Lanzhou, People’s Republic of China 2.University of Chinese Academy of Sciences, Beijing 100049, People’s Republic of China |
推荐引用方式 GB/T 7714 | Jin CY,Zeng FK,Liu G,et al. Recovery of Protease Inhibitors from Potato Fruit Water by Expanded Bed Adsorption Chromatography in Pilot Scale[J]. American Journal of Potato Research,2018,95(1):1-8. |
APA | Jin CY,Zeng FK,Liu G,&Ceng FK.(2018).Recovery of Protease Inhibitors from Potato Fruit Water by Expanded Bed Adsorption Chromatography in Pilot Scale.American Journal of Potato Research,95(1),1-8. |
MLA | Jin CY,et al."Recovery of Protease Inhibitors from Potato Fruit Water by Expanded Bed Adsorption Chromatography in Pilot Scale".American Journal of Potato Research 95.1(2018):1-8. |
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