Magnetic molecularly imprinted nanoparticles (MMIPs) with improved dispersity and an increased number of adsorption sites are described. Uniform silica layers were first deposited on the surface of Fe₃O₄ nanoparticles (Fe₃O₄ NPs) in order to improve the dispersity of magnetic nanoparticles. Then, 4-formylphenylboronic acid (FPBA) as functional monomer was immobilized on the magnetic carriers to improve the efficiency of template eluting and rebinding. A thin layer of polyaniline imprinted with horseradish peroxidase (HRP) as a model glycoprotein was then placed on the magnetic nanoparticles to enhance the dispersity of the resultant MMIPs. These exhibit high adsorption capacity (62 mg g⁻¹), a satisfactory imprinting factor ( 3.78) and short adsorption equilibrium time (40 min) toward HRP, and the limit of detection is 18.7 μg L⁻¹. This kind of MMIPs, therefore, is deemed being a useful tool for extracting low-abundance glycoproteins from even complex samples.