A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with apolyadenine-modified aptamer

doi:10.1007/s00604-018-3067-0

	A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with apolyadenine-modified aptamer
	Xie, Yuanyang 1,2; Huang, Yu1 ; Tang, Dongyun1 ; Cui, Hongliang1 ; Cao, Haiyan 3
刊名	MICROCHIMICA ACTA
	2018-12-01
卷号	185 期号:12 页码:9
关键词	D-(-)-threo-2-amino-1-(4-nitrophenyl)-1,3-propanediol Surface charge Water analysis
ISSN号	0026-3672
DOI	10.1007/s00604-018-3067-0
通讯作者	Huang, Yu(huangyu@cigit.ac.cn) ; Cao, Haiyan(513923170@qq.com)
英文摘要	A competitive colorimetric assay has been established to detect chloramphenicol (CAP). It is based on the use of colloidal and electrostatically stabilized aptamer-modified gold nanoparticles (GNPs). The CAP aptamer is modified by a sequence of 5 adenosine groups to anchor it on the surface of GNPs. It can competitively capture two compounds, viz. D-(-)-threo-2-amino-1-(4-nitrophenyl)-1,3-propanediol (CAP-base, with a positive charge) and CAP (which is uncharged). The capture of the positively charged CAP-base triggers the aggregation of modified GNPs in salt-containing solution, and this causes a color change from red to purple. However, in the presence of CAP and CAP-base, the capture of the uncharged CAP weakens this color change by a competing process for capture. Thus, the concentration of CAP is associated with the degree of deaggregation of GNPs and can be quantified by the ratio of absorbances at 620nm and 520nm. The assay has a 22nM limit of detection in acidic solution, and the response is linear in the range of 0.20 to 3.20M CAP concentration. This assay was successfully applied to the determination of CAP in spiked environmental water samples. Conceivably, this method has a wide scope in that it may be applied to a wide range of analytes if respective aptamers are available.
资助项目	National Natural Science Foundation of China[21707137] ; Key Application and Development Program of Chongqing[cstc2017shmsA0159] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0718] ; Research Funding Project of Yangtze Normal University[2017KYQD23] ; Young Scientist Research Fund of Yangtze Normal University ; Open Research Fund Program of Key Laboratory of Reservoir Aquatic Environment of CAS
WOS研究方向	Chemistry
语种	英语
出版者	SPRINGER WIEN
WOS记录号	WOS:000449518500002
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/6993]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Huang, Yu; Cao, Haiyan
作者单位	1.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Chongqing 400714, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yangtze Normal Univ, Sch Chem & Chem Engn, Chongqing 408100, Peoples R China
推荐引用方式 GB/T 7714	Xie, Yuanyang,Huang, Yu,Tang, Dongyun,et al. A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with apolyadenine-modified aptamer[J]. MICROCHIMICA ACTA,2018,185(12):9.
APA	Xie, Yuanyang,Huang, Yu,Tang, Dongyun,Cui, Hongliang,&Cao, Haiyan.(2018).A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with apolyadenine-modified aptamer.MICROCHIMICA ACTA,185(12),9.
MLA	Xie, Yuanyang,et al."A competitive colorimetric chloramphenicol assay based on the non-cross-linking deaggregation of gold nanoparticles coated with apolyadenine-modified aptamer".MICROCHIMICA ACTA 185.12(2018):9.