Copper-doped mesoporous hydroxyapatite microspheres synthesized by a microwave-hydrothermal method using creatine phosphate as an organic phosphorus source: application in drug delivery and enhanced bone regeneration

doi:10.1039/c6tb02747d

	Copper-doped mesoporous hydroxyapatite microspheres synthesized by a microwave-hydrothermal method using creatine phosphate as an organic phosphorus source: application in drug delivery and enhanced bone regeneration
	Yu, Weilin 1; Sun, Tuan-Wei 2,3; Ding, Zhenyu 1; Qi, Chao 2,3; Zhao, Huakun 1; Chen, Feng 2,3; Shi, Zhongmin 1; Zhu, Ying-Jie 2,3; Chen, Daoyun 1; He, Yaohua 1,4
刊名	Journal of Materials Chemistry B
	2017
卷号	5 期号:5 页码:1039-1052
ISSN号	20507518
DOI	10.1039/c6tb02747d
英文摘要	The development of multifunctional biomaterials with drug delivery ability, and pro-osteogenic and pro-angiogenic activities has garnered increasing interest in the field of regenerative medicine. In the present study, hypoxia-mimicking copper (Cu)-doped mesoporous hydroxyapatite (HAP) microspheres (Cu-MHMs) were successfully synthesized through a microwave-hydrothermal method by using creatine phosphate as an organic phosphorus source. The Cu-MHMs doped with 0.2, 0.5 and 1 mol% Cu were prepared. The Cu-MHMs consisting of HAP nanorods or nanosheets exhibited a hierarchically mesoporous hollow structure and a high specific surface area. Then the Cu-MHMs were investigated as a drug nanocarrier using doxorubicin hydrochloride (DOX) as a model drug. The Cu-MHMs showed a relatively high drug-loading capacity and a pH-responsive drug release behavior. Furthermore, the Cu-MHMs were incorporated into a chitosan (CS) matrix to construct a biomimetic scaffold optimized for bone regeneration. The Cu-MHM/CS composite scaffolds maintained high degrees of porosity and showed a sustained release of Cu ions. More importantly, the Cu-MHM/CS scaffolds not only enhanced the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells (rBMSCs) but also promoted the migration and tube formation of EA.hy926 cells. When implanted in rat critical-sized calvarial defects, the Cu-MHM/CS scaffolds significantly enhanced bone regeneration accompanied by more new blood vessel formation at 8 weeks post-operation compared with the MHM/CS scaffolds. These results suggest that the hypoxia-mimicking Cu-MHM/CS scaffolds could encourage bone regeneration by enhancing osteogenesis and angiogenesis simultaneously, which bodes well for the reconstruction of vascularized tissue-engineered bone. © The Royal Society of Chemistry.
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/25824]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai; 200233, China; 2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai; 200050, China; 3.University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing; 100049, China; 4.Shanghai Jiao Tong University Affiliated Sixth People's Hospital, School of Biomedical Engineering, 600 Yishan Road, Shanghai; 200233, China
推荐引用方式 GB/T 7714	Yu, Weilin,Sun, Tuan-Wei,Ding, Zhenyu,et al. Copper-doped mesoporous hydroxyapatite microspheres synthesized by a microwave-hydrothermal method using creatine phosphate as an organic phosphorus source: application in drug delivery and enhanced bone regeneration[J]. Journal of Materials Chemistry B,2017,5(5):1039-1052.
APA	Yu, Weilin.,Sun, Tuan-Wei.,Ding, Zhenyu.,Qi, Chao.,Zhao, Huakun.,...&He, Yaohua.(2017).Copper-doped mesoporous hydroxyapatite microspheres synthesized by a microwave-hydrothermal method using creatine phosphate as an organic phosphorus source: application in drug delivery and enhanced bone regeneration.Journal of Materials Chemistry B,5(5),1039-1052.
MLA	Yu, Weilin,et al."Copper-doped mesoporous hydroxyapatite microspheres synthesized by a microwave-hydrothermal method using creatine phosphate as an organic phosphorus source: application in drug delivery and enhanced bone regeneration".Journal of Materials Chemistry B 5.5(2017):1039-1052.