Enhanced osteogenic activity of anatase TiO2film: Surface hydroxyl groups induce conformational changes in fibronectin

doi:10.1016/j.msec.2017.04.056

	Enhanced osteogenic activity of anatase TiO2film: Surface hydroxyl groups induce conformational changes in fibronectin
	Lv, Lin 1,2; Li, Kai 1; Xie, Youtao 1; Cao, Yunzhen 1; Zheng, Xuebin 1
刊名	Materials Science and Engineering C
	2017
卷号	78 页码:96-104
ISSN号	09284931
DOI	10.1016/j.msec.2017.04.056
英文摘要	In this study, with an attempt to identify the effects of TiO2crystalline phase compositions on the osteogenic properties, the anatase and rutile TiO2thin films with similar film thickness, surface topography and hydrophilicity were prepared on Si (100) substrates by atomic layer deposition (ALD), subsequent thermal annealing and ultraviolet irradiation. The films were studied with XRD, XPS, FE-SEM, AFM, FTIR and contact angle measurements. In vitro cellular assays showed that the anatase phase led to better osteoblast compatibility in terms of adhesion, proliferation, differentiation, mineralization as well as osteogenesis-related gene expression when compared with the rutile phase. We investigated the difference between the anatase and rutile TiO2films at the biomolecular level to explain the enhanced osteogenic activity of the anatase film. It was found that the presence of more Ti[sbnd]OH groups on anatase surface induced more cell-binding sites of fibronectin (FN) exposed on its surface, causing a more active conformation of the adsorbed FN for subsequent osteoblast behaviors. © 2017
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/25866]
专题	中国科学院上海硅酸盐研究所
通讯作者	Cao, Yunzhen
作者单位	1.Key Laboratory of Inorganic Coating Materials, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai; 200050, China; 2.University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing; 100049, China
推荐引用方式 GB/T 7714	Lv, Lin,Li, Kai,Xie, Youtao,et al. Enhanced osteogenic activity of anatase TiO2film: Surface hydroxyl groups induce conformational changes in fibronectin[J]. Materials Science and Engineering C,2017,78:96-104.
APA	Lv, Lin,Li, Kai,Xie, Youtao,Cao, Yunzhen,&Zheng, Xuebin.(2017).Enhanced osteogenic activity of anatase TiO2film: Surface hydroxyl groups induce conformational changes in fibronectin.Materials Science and Engineering C,78,96-104.
MLA	Lv, Lin,et al."Enhanced osteogenic activity of anatase TiO2film: Surface hydroxyl groups induce conformational changes in fibronectin".Materials Science and Engineering C 78(2017):96-104.