Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen

doi:10.1021/acs.molpharmaceut.8b00722

	Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen
	Liu, Q; Chen, XM; Jia, JL; Lu, T; Yang, TY; Wang, LY; Liu, Qi; Chen, Xiaoming; Jia, Jilei; Lu, Ting
刊名	MOLECULAR PHARMACEUTICS
	2018
卷号	15 期号:11 页码:5227
关键词	DDAB-PLA MEDIATED IMMUNE-RESPONSES microparticles IFN-GAMMA HBsAg IN-VIVO antibody PARTICULATE VACCINES cellular response ANTIBODY-RESPONSES ALUM ADJUVANT GRANZYME-B NANOPARTICLES DELIVERY PROTEINS
ISSN号	1543-8384
DOI	10.1021/acs.molpharmaceut.8b00722
文献子类	Article
英文摘要	For the purpose of strengthening the immunogenicity of the hepatitis B vaccine, which contains hepatitis B surface antigen (HBsAg), the development of biodegradable poly(lactic acid) (PLA) microparticles (MPs) modified with the cationic surfactant didodecyldimethylammonium bromide (DDAB) was attempted. DDAB-PLA MPs with an uniform size of about 1 mu m were prepared in a simple and mild way. DDAB-PLA MPs with increased surface charge enhanced antigen adsorption capacity compared to plain PLA MPs. After immunization, DDAB-PLA MPs induced the gene expression of inflammatory cytokines and chemokines, which facilitated the following immune responses. DDAB-PLA MPs augmented the expression of co-stimulatory molecules along with the activation of bone-marrow-derived dendritic cells (BMDCs). DDAB-PLA MP-based vaccine formulations efficiently induced antibody production more than the aluminum-based vaccine and plain PLA MP-based formulation in vivo. Moreover, DDAB-PLA MPs were more likely to generate the polarization of the Th1 response indicating the cytotoxic ability against infectious pathogens. In conclusion, DDAB-PLA MPs could be a potent vaccine formulation to prime robust cellular and humoral immune responses.
WOS记录号	WOS:000449577000039
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/26782]
专题	中国科学院过程工程研究所
推荐引用方式 GB/T 7714	Liu, Q,Chen, XM,Jia, JL,et al. Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen[J]. MOLECULAR PHARMACEUTICS,2018,15(11):5227, 5235.
APA	Liu, Q.,Chen, XM.,Jia, JL.,Lu, T.,Yang, TY.,...&Wang, Lianyan.(2018).Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen.MOLECULAR PHARMACEUTICS,15(11),5227.
MLA	Liu, Q,et al."Potential Hepatitis B Vaccine Formulation Prepared by Uniform-Sized Lipid Hybrid PLA Microparticles with Adsorbed Hepatitis B Surface Antigen".MOLECULAR PHARMACEUTICS 15.11(2018):5227.