Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose
Zhu, Chang-hui; Fang, Zhen; Su, Tong-chao; Li, Xing-kang; Liu, Qi-ying
刊名CELLULOSE
2018
卷号25期号:4页码:2473-2485
关键词Mesoporous Biochar Ionothermal Carbonization Immobilized Cellulase Kinetics Analysis Stability
ISSN号0969-0239
英文摘要Cellulose-based biochar was prepared via ionothermal carbonization of cellulose in [Bmim]Cl with H(2)SO(4)and subsequent pyrolysis. The biochar was analyzed by a series of characterization methods, indicating that it was a kind of mesoporous carbon suitable for the adsorption of cellulase. Kinetic analysis showed that the immobilized cellulase exhibited higher affinity to carboxymethyl cellulose than free cellulase. The immobilized cellulase, at different pH and temperatures, was more stable than free cellulase. It was used to hydrolyze pretreated cellulose in [Bmim]Cl with a total reducing sugar (TRS) yield of 99.9%. The immobilized cellulase maintained activity of 74.8% after five cycles at an immobilized cellulase/cellulose weight ratio of 30:1. When the cellulose loading was increased by a factor of 5, the TRS yield decreased by only 27.5%.
学科主题Materials Science ; Polymer Science
URL标识查看原文
语种英语
公开日期2018-05-10
内容类型期刊论文
源URL[http://ir.xtbg.org.cn/handle/353005/10849]  
专题西双版纳热带植物园_生物能源研究组
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GB/T 7714
Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,et al. Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose[J]. CELLULOSE,2018,25(4):2473-2485.
APA Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,Li, Xing-kang,&Liu, Qi-ying.(2018).Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose.CELLULOSE,25(4),2473-2485.
MLA Zhu, Chang-hui,et al."Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose".CELLULOSE 25.4(2018):2473-2485.
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