De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 能源植物分子育种研究组

	De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis
	Fu, Qiantang; Niu, Longjian; Chen, Mao-Sheng; Tao, Yan-Bin; Wang, Xiulan; He, Huiying; Pan, Bang-Zhen; Xu, Zeng-Fu
刊名	JOURNAL OF PLANT PHYSIOLOGY
	2018
卷号	221 期号:x 页码:107-118
关键词	Promising Oilseed Crop Time Quantitative Pcr Mads-box Genes Flower Development Sex Determination Arabidopsis-thaliana Silene-latifolia Ricinus-communis Expression Plants
ISSN号	0176-1617
英文摘要	Plukenetia volubilis is a promising oilseed crop due to its seeds being rich in unsaturated fatty acids, especially alpha-linolenic acid. P. volubilis is monoecious, with separate male and female flowers on the same inflorescence. We previously reported that male flowers were converted to female flowers by exogenous cytokinin (6-benzyladenine, 6-BA) treatment in P. volubilis. To identify candidate genes associated with floral sex differentiation of P. volubilis, we performed de novo transcriptome assembly and comparative analysis on control male inflorescence buds (MIB) and female inflorescence buds (FIB) induced by 6-BA using Illumina sequencing technology. A total of 57,664 unigenes with an average length of 979 bp were assembled from 104.1 million clean reads, and 45,235 (78.45%) unigenes were successfully annotated in the public databases. Notably, Gene Ontology analyses revealed that 4193 and 3880 unigenes were enriched in the categories of reproduction and reproductive processes, respectively. Differential expression analysis identified 1385 differentially expressed unigenes between MIB and FIB, of which six unigenes related to cytokinin and auxin signaling pathways and 16 important transcription factor (TF) genes including MADS-box family members were identified. In particular, several unigenes encoding important TFs, such as homologs of CRABS CLAW, RADIALIS-like 1, RADIALIS-like 2, HECATE 2, WUSCHEL-related homeobox 9, and SUPERMAN, were expressed at higher levels in FIB than in MIB. The expression patterns of the 36 selected unigenes revealed by transcriptome analysis were successfully validated by quantitative real-time PCR. This study not only provides comprehensive gene expression profiles of P. volubilis inflorescence buds, but also lays the foundation for research on the molecular mechanism of floral sex determination in P. volubilis and other monoecious plants.
学科主题	Plant Sciences
URL标识	查看原文
语种	英语
公开日期	2018-03-07
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10792]
专题	西双版纳热带植物园_能源植物分子育种研究组
通讯作者	Xu, Zeng-Fu
推荐引用方式 GB/T 7714	Fu, Qiantang,Niu, Longjian,Chen, Mao-Sheng,et al. De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis[J]. JOURNAL OF PLANT PHYSIOLOGY,2018,221(x):107-118.
APA	Fu, Qiantang.,Niu, Longjian.,Chen, Mao-Sheng.,Tao, Yan-Bin.,Wang, Xiulan.,...&Xu, Zeng-Fu.(2018).De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis.JOURNAL OF PLANT PHYSIOLOGY,221(x),107-118.
MLA	Fu, Qiantang,et al."De novo transcriptome assembly and comparative analysis between male and benzyladenine-induced female inflorescence buds of Plukenetia volubilis".JOURNAL OF PLANT PHYSIOLOGY 221.x(2018):107-118.