A redox cycling-amplified electrochemical immunosensor for -fetoprotein sensitive detection via polydopamine nanolabels

doi:10.1039/c8nr02946f

	A redox cycling-amplified electrochemical immunosensor for -fetoprotein sensitive detection via polydopamine nanolabels
	Xiang, Haipeng 1; Wang, Yunli ; Wang, Ming ; Shao, Yiran ; Jiao, Yajing ; Zhu, Yingchun
刊名	NANOSCALE
	2018
卷号	10 期号:28 页码:13572
ISSN号	2040-3364
DOI	10.1039/c8nr02946f
英文摘要	A sandwich-type electrochemical immunosensor for sensitive detection of a tumor marker, -fetoprotein (AFP), was fabricated by employing polydopamine-detection antibody nanoparticles (PDANPs-Ab(2)) as selective redox cycling-based signal amplifiers on an electrodeposited nano-gold electrode. In this research, PDANPs prepared through oxidative polymerization of dopamine were found to amplify the oxidation charge transfer of the electrochemical mediator (1,1-ferrocene dimethanol, FDM), which was supported by cyclic voltammetry (CV) and X-ray photoelectron spectroscopy (XPS) investigation. Therefore, PDANPs were utilized as label materials of electrochemical immunosensors to enhance sensitivity for the first time. Meanwhile, the nano-gold electrode was used as a platform to accelerate electron transfer and immobilize capture antibody (Ab(1)). The electrochemical performance of the AFP immunosensor was investigated in PBS containing FDM with CV. Under optimal conditions, the constructed AFP immunosensor exhibited a wide linear range from 1 pg mL(-1) to 50 ng mL(-1) and a low detection limit of 0.3 pg mL(-1), as well as excellent stability, reproducibility and selectivity. Measurements of AFP in human serum gave excellent correlation with the clinical standard Chemiluminescence Microparticle Immuno Assay (CMIA). These results indicated that the developed immunosensor may have promising application in the clinical diagnosis of AFP and other tumor markers.
学科主题	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000439319000031
资助机构	This work was sponsored by the Shanghai Sailing Program (No. 17YF1421700), and supported by the National Natural Science Foundation of China (No. 51572283 and 51232007), the Science and Technology Commission of Shanghai Municipality (No. 18441900800, No. 08JC1420700 and No. 11XD1405600) and the Innovation Fund of SICCAS (No. Y75ZC8180G and No. Y85ZC8180G). ; This work was sponsored by the Shanghai Sailing Program (No. 17YF1421700), and supported by the National Natural Science Foundation of China (No. 51572283 and 51232007), the Science and Technology Commission of Shanghai Municipality (No. 18441900800, No. 08JC1420700 and No. 11XD1405600) and the Innovation Fund of SICCAS (No. Y75ZC8180G and No. Y85ZC8180G).
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24805]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, Key Lab Inorgan Coating Mat CAS, Shanghai 200050, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiang, Haipeng,Wang, Yunli,Wang, Ming,et al. A redox cycling-amplified electrochemical immunosensor for -fetoprotein sensitive detection via polydopamine nanolabels[J]. NANOSCALE,2018,10(28):13572, 13580.
APA	Xiang, Haipeng,Wang, Yunli,Wang, Ming,Shao, Yiran,Jiao, Yajing,&Zhu, Yingchun.(2018).A redox cycling-amplified electrochemical immunosensor for -fetoprotein sensitive detection via polydopamine nanolabels.NANOSCALE,10(28),13572.
MLA	Xiang, Haipeng,et al."A redox cycling-amplified electrochemical immunosensor for -fetoprotein sensitive detection via polydopamine nanolabels".NANOSCALE 10.28(2018):13572.