3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities

doi:10.7150/thno.27088

	3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities
	Dang, Wentao 1; Wang, Xiaoya ; Li, Jiayi 2; Deng, Cuijun 1; Liu, Yaqin 1; Yao, Qingqiang 2; Wang, Liming 2; Chang, Jiang ; Wu, Chengtie
刊名	THERANOSTICS
	2018
卷号	8 期号:16 页码:4372
ISSN号	1838-7640
DOI	10.7150/thno.27088
英文摘要	When osteochondral tissues suffer from focal or degenerative lesions caused by trauma or disorders, it is a tough challenge to regenerate them because of the limited self-healing capacity of articular cartilage. In this study, a series of Mo-doped bioactive glass ceramic (Mo-BGC) scaffolds were prepared and then systematically characterized. The released MoO42-ions from 7.5Mo-BGC scaffolds played a vital role in regenerating articular cartilage and subchondral bone synchronously. Methods: The Mo-BGC scaffolds were fabricated through employing both a sol-gel method and 3D printing technology. SEM, EDS, HRTEM, XRD, ICPAES and mechanical strength tests were respectively applied to analyze the physicochemical properties of Mo-BGC scaffolds. The proliferation and differentiation of rabbit chondrocytes (RCs) and human bone mesenchymal stem cells (HBMSCs) cultured with dilute solutions of 7.5Mo-BGC powder extract were investigated in vitro. The co-culture model was established to explore the possible mechanism of stimulatory effects of MoO42-ions on the RCs and HBMSCs. The efficacy of regenerating articular cartilage and subchondral bone using 7.5Mo-BGC scaffolds was evaluated in vivo. Results: The incorporation of Mo into BGC scaffolds effectively enhanced the compressive strength of scaffolds owing to the improved surface densification. The MoO42-ions released from the 7.5Mo-BGC powders remarkably promoted the proliferation and differentiation of both RCs and HBMSCs. The MoO42-ions in the co-culture system significantly stimulated the chondrogenic differentiation of RCs and meanwhile induced the chondrogenesis of HBMSCs. The chondrogenesis stimulated by MoO42-ions happened through two pathways: 1) MoO42-ions elicited anabolic responses through activating the HIF-1 alpha signaling pathway; 2) MoO42-ions inhibited catabolic responses and protected cartilage matrix from degradation. The in vivo study showed that 7.5Mo-BGC scaffolds were able to significantly promote cartilage/bone regeneration when implanted into rabbit osteochondral defects for 8 and 12 weeks, displaying bi-lineage bioactivities. Conclusion: The 3D-printed Mo-BGC scaffolds with bi-lineage bioactivities and activated anabolic responses could offer an effective strategy for cartilage/bone interface regeneration.
学科主题	Medicine, Research & Experimental
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000444104300008
资助机构	This work was supported by the National Key Research and Development Program of China (2018YFB1105501), the Natural Science Foundation of China (5171101275, 81771989, 81601612), Key Research Program of Frontier Sciences CAS (QYZDB-SSW-SYS027) and Science and Technology Commission of Shanghai Municipality (17441903700, 16DZ2260603, 17540712300) and Key Research Program of Science & Technology Support Program of Jiangsu Province (BE2016763). ; This work was supported by the National Key Research and Development Program of China (2018YFB1105501), the Natural Science Foundation of China (5171101275, 81771989, 81601612), Key Research Program of Frontier Sciences CAS (QYZDB-SSW-SYS027) and Science and Technology Commission of Shanghai Municipality (17441903700, 16DZ2260603, 17540712300) and Key Research Program of Science & Technology Support Program of Jiangsu Province (BE2016763).
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24561]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Nanjing Med Univ, Nanjing Hosp, Digital Med Inst, Dept Orthopaed Surg, 68 Changle Rd, Nanjing 210006, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Dang, Wentao,Wang, Xiaoya,Li, Jiayi,et al. 3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities[J]. THERANOSTICS,2018,8(16):4372, 4392.
APA	Dang, Wentao.,Wang, Xiaoya.,Li, Jiayi.,Deng, Cuijun.,Liu, Yaqin.,...&Wu, Chengtie.(2018).3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities.THERANOSTICS,8(16),4372.
MLA	Dang, Wentao,et al."3D printing of Mo-containing scaffolds with activated anabolic responses and bi-lineage bioactivities".THERANOSTICS 8.16(2018):4372.