Sr-doped nanowire modification of Ca-Si-based coatings for improved osteogenic activities and reduced inflammatory reactions

doi:10.1088/1361-6528/aaa2b4

	Sr-doped nanowire modification of Ca-Si-based coatings for improved osteogenic activities and reduced inflammatory reactions
	Li, Kai; Hu, Dandan; Xie, Youtao; Huang, Liping; Zheng, Xuebin
刊名	NANOTECHNOLOGY
	2018
卷号	29 期号:8
关键词	Ca-Si coatings nanowire-like topography Sr dopant osteogenic activities inflammatory reactions
ISSN号	0957-4484
DOI	10.1088/1361-6528/aaa2b4
英文摘要	Biomedical coatings for orthopedic implants should facilitate osseointegration and mitigate implant-induced inflammatory reactions. In our study, Ca-Si coatings with Sr-containing nanowire-like structures (NW-Sr-CS) were achieved via hydrothermal treatment. In order to identify the effect of nanowire-like topography and Sr dopant on the biological properties of Ca-Si-based coatings, the original Ca-Si coating, Ca-Si coatings modified with nanoplate (NP-CS) and similar nanowire-like structure (NW-CS) were fabricated as the control. Surface morphology, phase composition, surface area, zeta potential and ion release of these coatings were characterized. The in vitro osteogenic activities and immunomodulatory properties were evaluated with bone marrow stromal cells (BMSCs) and RAW 264.7 cells, a mouse macrophage cell line. Compared with the CS and NP-CS coatings, the NW-CS coating possessed a larger surface area and pore volume, beneficial protein adsorption, up-regulated the expression levels of integrin beta 1, Vinculin and focal adhesion kinase and promoted cell spreading. Furthermore, the NW-CS coating significantly enhanced the osteogenic differentiation and mineralization as indicated by the up-regulation of ALP activity, mineralized nodule formation and osteoblastogenesis-related gene expression. With the introduction of Sr, the NW-Sr-CS coatings exerted a greater effect on the BMSC proliferation rate, calcium sensitive receptor gene expression as well as PKC and ERK1/2 phosphorylation. In addition, the Sr-doped coatings significantly up-regulated the ratio of OPG/RANKL in the BMSCs. The NW-Sr-CS coatings could modulate the polarization of macrophages towards the wound-healing M2 phenotype, reduce the mRNA expression levels of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6) and enhance anti-inflammatory cytokines (IL-1ra, IL-10). The Sr-doped nanowire modification may be a valuable approach to enhance osteogenic activities and reduce inflammatory reactions.
学科主题	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
出版者	IOP PUBLISHING LTD
WOS记录号	WOS:000423021600001
资助机构	This work was supported by the National Natural Science Foundation of China (Grant No 51502328) and the Shanghai Science and Technology Committee (Grant No 17441904400). ; This work was supported by the National Natural Science Foundation of China (Grant No 51502328) and the Shanghai Science and Technology Committee (Grant No 17441904400).
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24460]
专题	中国科学院上海硅酸盐研究所
作者单位	Chinese Acad Sci, Shanghai Inst Ceram, Key Lab Inorgan Coating Mat CAS, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Li, Kai,Hu, Dandan,Xie, Youtao,et al. Sr-doped nanowire modification of Ca-Si-based coatings for improved osteogenic activities and reduced inflammatory reactions[J]. NANOTECHNOLOGY,2018,29(8).
APA	Li, Kai,Hu, Dandan,Xie, Youtao,Huang, Liping,&Zheng, Xuebin.(2018).Sr-doped nanowire modification of Ca-Si-based coatings for improved osteogenic activities and reduced inflammatory reactions.NANOTECHNOLOGY,29(8).
MLA	Li, Kai,et al."Sr-doped nanowire modification of Ca-Si-based coatings for improved osteogenic activities and reduced inflammatory reactions".NANOTECHNOLOGY 29.8(2018).