Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe

doi:10.1039/c8cc07605g

	Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe
	Liu, Maili 3; Zhang, Xu 3; Cao, Chunyang 1; Lan, Wenxian 1; Li, Conggang 3; Jiang, Bin 3; Zhu, Qinjun 3; Yuan, Bin 3; Wang, Qianwen 2,3; Sun, Peng 3
刊名	CHEMICAL COMMUNICATIONS
	2018-11-18
卷号	54 期号:89 页码:12630-12633
ISSN号	1359-7345
DOI	10.1039/c8cc07605g
英文摘要	Spectral overlap makes it difficult to use NMR for mapping the conformational profile of heterogeneous conformational ensembles of macromolecules. Here, we apply a H-1-N-14 HSQC experiment to monitor the alkaline conformational transitions of yeast iso-1 cytochrome c (ycyt c) at natural isotopic abundance. Trimethylated Lys72 of ycyt c is selectively detected by a H-1-N-14 HSQC experiment, and used as a probe to trace conformational transitions of ycyt c under alkaline conditions. It was found that at least four different conformers of ycyt c coexisted under alkaline conditions. Besides the native structure, Lys73 or Lys79 coordinated conformers and a partially unfolded state with exposed heme were observed. These results indicate that the method is powerful at simplifying spectra of a trimethylated protein, which makes it possible to study complex conformational transitions of naturally extracted or chemically modified trimethylated protein at natural isotopic abundance.
资助项目	National Key R&D Program of China[2017YFA0505400] ; National Natural Science Foundation of China[21735007] ; National Natural Science Foundation of China[21505153] ; National Natural Science Foundation of China[21675170] ; National Natural Science Foundation of China[21475146] ; National Natural Science Foundation of China[21773300] ; CAS Key Research Program of Frontier Sciences[QYZDJ-SSW-SLH027] ; K. C. Wong Educational Foundation
WOS关键词	CHOLINE-CONTAINING COMPOUNDS ; FERRICYTOCHROME-C ; LIVING CELLS ; CONFORMATIONAL TRANSITIONS ; PEROXIDASE-ACTIVITY ; SPECTROSCOPY ; PROTEINS ; STATE ; ISO-1-CYTOCHROME-C ; IDENTIFICATION
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000449274400015
资助机构	National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation ; National Key R&D Program of China ; National Natural Science Foundation of China ; CAS Key Research Program of Frontier Sciences ; K. C. Wong Educational Foundation
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13200]
专题	中国科学院武汉物理与数学研究所
通讯作者	Liu, Maili; Zhang, Xu
作者单位	1.Chinese Acad Sci, Shanghai Inst Organ Chem, Shanghai 200032, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, CAS Key Lab Magnet Resonance Biol Syst, Wuhan Natl Ctr Magnet Resonance,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Liu, Maili,Zhang, Xu,Cao, Chunyang,et al. Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe[J]. CHEMICAL COMMUNICATIONS,2018,54(89):12630-12633.
APA	Liu, Maili.,Zhang, Xu.,Cao, Chunyang.,Lan, Wenxian.,Li, Conggang.,...&Sun, Peng.(2018).Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe.CHEMICAL COMMUNICATIONS,54(89),12630-12633.
MLA	Liu, Maili,et al."Monitoring alkaline transitions of yeast iso-1 cytochrome c at natural isotopic abundance using trimethyllysine as a native NMR probe".CHEMICAL COMMUNICATIONS 54.89(2018):12630-12633.