Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2018专题

	Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer
	Dai, Qi; Jin, Yin-Hua; Hua, Qi-Feng; Zheng, Jian-Jun; Ma, Xue-Hua; Chen, Tian-Xiang; Zhang, Shun; Chen, Bin; Zhang, Xiao-Hui
刊名	CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
	2018
卷号	49 期号:1 页码:271-281
关键词	Growth-factor Receptor-2 Estrogen-receptor Progesterone-receptor In-vivo Folate Receptor Postmenopausal Women Expression Subtypes Cells Chemotherapy
英文摘要	Background/Aims: Smart molecular probes are required in the application of Magnetic resonance imaging (MRI) for biochemical and clinical research. This study aims to investigate the diagnostic values of estrogen receptor (ER), progesterone receptor (PR), folate receptor (FR) and human epidermal growth factor receptor 2 (HER-2)-targeted molecular probes in the MRI diagnosis of breast cancer. Methods: Initially, a total of 508 female breast cancer patients were selected for breast cancer subtype classification by immunohistochemistry. Subsequently, the tumor size, lymph node metastasis, and histological grade of different breast cancer subtypes were compared. Molecular probes of Ab-ER-USPIO, Ab-PR-USPIO, Ab-FR-USPIO and Ab-HER-2-USPIO were constructed and screened. The specific binding of molecular probes to breast cancer cells was detected both in vitro and in vivo by Prussian blue staining and MRI using T1 and T2 weighted images. Finally, in vivo toxicity of Ab-HER-2-USPIO was analyzed using hematoxylin and eosin staining. Results: We identified the following subtypes of breast cancer: Luminal A (ER-positive, FR-positive, HER-2-negative), Luminal B (ER-positive, FR-positive, HER-2-positive), HER-2 overexpression (ER-negative, FR-negative, HER-2-positive), and triple-negative breast cancer (ER-negative, FR-negative, HER-2-negative). Featuring favorable in vitro biocompatibility and low in vivo toxicity, Ab-HER-2-USPIO can specifically bind to breast cancer cells BT47 and SKBR3, thus enhancing the quality of T1 weighted MRI images. Conclusion: The results indicate that HER-2-targeted MRI molecular probes may be used in the clinical diagnosis of breast cancer and facilitate the development of promising strategies for breast cancer treatments. (C) 2018 The Author(s) Published by S. Karger AG, Basel.
学科主题	Polymer Science
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/16889]
专题	2018专题
推荐引用方式 GB/T 7714	Dai, Qi,Jin, Yin-Hua,Hua, Qi-Feng,et al. Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer[J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,2018,49(1):271-281.
APA	Dai, Qi.,Jin, Yin-Hua.,Hua, Qi-Feng.,Zheng, Jian-Jun.,Ma, Xue-Hua.,...&Zhang, Xiao-Hui.(2018).Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer.CELLULAR PHYSIOLOGY AND BIOCHEMISTRY,49(1),271-281.
MLA	Dai, Qi,et al."Diagnostic Value of ER, PR, FR and HER-2-Targeted Molecular Probes for Magnetic Resonance Imaging in Patients with Breast Cancer".CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 49.1(2018):271-281.