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题名	拉曼光谱探测相关技术研究
作者	戴艳
文献子类	硕士
导师	董作人
关键词	拉曼光谱 Raman spectroscopy 拉曼探头 Raman probe 双轴共焦 biaxial confocal 荧光干扰 fluorescence interference 微型光谱仪 micro spectrometer
其他题名	Study on relevant technology of Raman spectrum detection
英文摘要	拉曼光谱检测技术具有可无损检测、微量检测等诸多优点，在医学、生物、化学、食品安全等领域应用广泛。但拉曼检测过程中，不可避免会产生荧光，其强度甚至可达拉曼光的106~108倍，很大程度上影响了拉曼信号的识别。因此研究拉曼光谱检测中的荧光抑制技术很有必要。本论文对拉曼光谱探测技术尤其是荧光抑制技术做了深入分析，提出了一种用以抑制样品装载容器的荧光的双轴共焦检测技术。除此之外，还研制了拉曼光谱探测关键部件——双光纤拉曼探测部件，及对微型光谱仪进行了相关研究。主要内容如下: 1、为消除拉曼光谱检测中样品装载容器的荧光背景干扰，利用样品拉曼信号光与容器所产生的荧光不共焦的特性，设计了一种双轴共焦的检测光路结构。实验结果表明，来自容器的荧光干扰幅度有数量级的衰减，拉曼信号光幅度下降了约30%。双轴共焦拉曼检测方法能够避免传统共轴共焦检测方法中拉曼信号光和荧光混合接收的问题，实现了对荧光材料容器装载的样品拉曼信号的有效检测。 2、在对传统共轴共焦拉曼光谱信号探测部件进行了结构和光路分析的基础上，设计了一种双光纤结构的拉曼探头，搭建了高精度收发共轴拉曼探头装配平台，所研制的双光纤拉曼探头与市场上同类产品相比，具有更高的激励光出光效率，更好的稳定性和一致性。 3、分析研究了微型拉曼光谱仪的原理和结构，利用Embed光谱仪的交叉C-T光机结构件，开展了基于高速线阵CCD（ILX511B）的拉曼光谱信号探测和波长校准技术研究，研制了基于DSP（TMS320F2812PGFA）的线阵CCD图像信号数据采集板，开发了基于Labview的线阵CCD上位机测控软件，实现了对拉曼光谱信号的准确探测。; Because Raman spectroscopy detection technology has many advantages such as nondestructive and convenience. it is widely used in medical, biological, chemical, food safety and other fields. However, Raman detection process will inevitably excite fluorescence, and the intensity of fluorescence can even reach 106~108 times as much as that of Raman light, which greatly affects the identification of Raman signals. Therefore, it is necessary to study the fluorescence suppression technology in Raman spectroscopy detection. In this dissertation, the Raman spectroscopy detection technology, especially the fluorescence suppression technology, is deeply analyzed. A biaxial confocal detection technique is proposed to suppress the fluorescence of the container of the sample. In addition, As a key component of Raman spectrum detection, the dual-fiber Raman detection unit has also been developed. What’s more, the related research has been conducted on miniature spectrometers. The main contents are as follows: 1. Aimed at eliminating the fluorescence interference from containers of the sample in the detection of Raman spectroscopy, a dual-axis confocal detection scheme is proposed based on the characteristic that the fluorescence generated by the container and the Raman signal of the sample are not confocal. The experimental results show that the fluorescence interference signals from the container is reduced by an order of magnitude, and the intensity of Raman signal is only declined about 30%. The dual-axis confocal Raman detection scheme can solve the mixed reception problem of Raman signal and fluorescence in the conventional coaxial confocal Raman detection scheme, and achieve the effective detection of Raman signal of the material in the fluorescent material container finally. 2. Based on the structure and optical path analysis of the signal detection components of traditional coaxial confocal Raman spectrometers, a dual-fiber Raman probe is designed and a high- precision transceiving coaxial Raman probe assembly platform is set up. The developed dual-fiber Raman probe has higher excitation light output efficiency, better stability and consistency than similar products on the market. 3. The principle and structure of the miniature Raman spectrometer is analyzed and studied. Using the cross-section CT machine structure of the Embed spectrometer, the Raman spectroscopy signal detection and wavelength calibration technology based on the high speed linear array CCD (ILX511B) are studied and developed. The linear array CCD image signal data acquisition board based on DSP (TMS320F2812PGFA) chip is designed, and the host computer monitoring and control software based on Labview software is developed, which achieve accurate detection of Raman spectral signals.
学科主题	光学工程
内容类型	学位论文
源URL	[http://ir.siom.ac.cn/handle/181231/31082]
专题	中国科学院上海光学精密机械研究所
作者单位	中国科学院上海光学精密机械研究所
推荐引用方式 GB/T 7714	戴艳. 拉曼光谱探测相关技术研究[D].

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