Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores | |
Lan Meng-Fei1; An Ning1; Zhao Ying1; Cao Wei1; Li Heng1; Zhao Lan2; Huang Yong-Dong2; Zhang Rong-Yue1 | |
刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2018-02-01 | |
卷号 | 46期号:2页码:288-292 |
关键词 | Porogen Solubility Parameter Macroporous Microspheres Protein Separation |
ISSN号 | 0253-3820 |
DOI | 10.11895/j.issn.0253-3820.171299 |
文献子类 | Article |
英文摘要 | The macroporous microspheres were prepared through suspension polymerization and based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate. The effect of porogen on the microspheres structure was evaluated in terms of pore size and surface area. Porogen contained dichloromethane (delta = 9.7 (cal/cm(3))(1/2)) and N-octanol (delta = 10.3 (cal/cm(3))(1/2)) which corresponded to a good and poor solvent, respectively. The solubility parameter of porogen was controlled in the range of 9.89-10.09 (cal/cm(3))(1/2). The pore size of microspheres increased with the difference value of solubility parameter between the polymer and the porogen. On the contrary, the surface area of microspheres decreased in this study. The anion exchange media was prepared through coupling poly(ethylene imine) in the microspheres, and the proteins transport was determined by frontal analysis method. The macroporous microspheres with 257 nm pore size could still afford a high proteins capacity (45.1 mg/mL). These macroporous supports showed a large potential in a rapid separation of proteins. |
WOS关键词 | MICELLES SWELLING METHOD ; PROTEIN CHROMATOGRAPHY ; MICROSPHERES ; ADSORPTION ; MEDIA ; MODE |
WOS研究方向 | Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000427611700020 |
资助机构 | Beijing Natural Science Foundation, China(2162013) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/24106] ![]() |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Beijing Inst Petrochem Technol, Coll Chem Engn, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Lan Meng-Fei,An Ning,Zhao Ying,et al. Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(2):288-292. |
APA | Lan Meng-Fei.,An Ning.,Zhao Ying.,Cao Wei.,Li Heng.,...&Zhang Rong-Yue.(2018).Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(2),288-292. |
MLA | Lan Meng-Fei,et al."Effect of Porogen Solubility Parameter on Structure of Chromatographic Supports with Large Pores".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.2(2018):288-292. |
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