Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis | |
Chen, Huayou1,2; Ullah, Jawad1; Jia, Jinru1 | |
刊名 | JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY |
2017 | |
卷号 | 27期号:3页码:159-167 |
关键词 | Bacillus Subtilis Spore Bioremediation Biocatalysis Surface Display Vaccine Development |
ISSN号 | 1464-1801 |
DOI | 10.1159/000475177 |
文献子类 | Review |
英文摘要 | Spore surface display is the most desirable with enhanced effects, low cost, less time consuming and the most promising technology for environmental, medical, and industrial development. Spores have various applications in industry due to their ability to survive in harsh industrial processes including heat resistance, alkaline tolerance, chemical tolerance, easy recovery, and reusability. Yeast and bacteria, including gram-positive and -negative, are the most frequently used organisms for the display of various proteins (eukaryotic and prokaryotic), but unlike spores, they can rupture easily due to nutritive properties, susceptibility to heat, pH, and chemicals. Hence, spores are the best choice to avoid these problems, and they have various applications over nonspore formers due to amenability for laboratory purposes. Various strains of Clostridium and Bacillus are spore formers, but the most suitable choice for display is Bacillus subtilis because, according to the WHO, it is safe to humans and considered as "GRAS" (generally recognized as safe). This review focuses on the application of spore surface display towards industries, vaccine development, the environment, and peptide library construction, with cell surface display for enhanced protein expression and high enzymatic activity. Different vectors, coat proteins, and statistical analyses can be used for linker selection to obtain greater expression and high activity of the displayed protein. (C) 2017 S. Karger AG, Basel |
WOS关键词 | Cotb Anchor Protein ; Cell-surface ; Fusion Partner ; System ; Coat ; Expression ; Bacterium ; Antigens ; Enzymes ; Carrier |
WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000409331400003 |
资助机构 | National Key Basic Research Program of China (973 Program)(2011CBA00800) ; National Key Laboratory of Biochemical Engineering ; Key Agriculture Support Project of Jiangsu Province, PR China(BE2013400) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/23207] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Jiangsu Univ, Inst Life Sci, Zhenjiang 21200, Jiangsu, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Huayou,Ullah, Jawad,Jia, Jinru. Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis[J]. JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,2017,27(3):159-167. |
APA | Chen, Huayou,Ullah, Jawad,&Jia, Jinru.(2017).Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis.JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY,27(3),159-167. |
MLA | Chen, Huayou,et al."Progress in Bacillus subtilis Spore Surface Display Technology towards Environment, Vaccine Development, and Biocatalysis".JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY 27.3(2017):159-167. |
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