Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1

doi:10.1002/elsc.201700209

	Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1
	Yu, Haifeng 1,2; Liu, Bin 1,2; Luo, Jianquan 1,3; Cao, Weifeng 1; Qiao, Changsheng 4; Wan, Yinhua 1,3
刊名	ENGINEERING IN LIFE SCIENCES
	2018-06-01
卷号	18 期号:6 页码:379-386
关键词	Aureobasidium pullulans Biosynthesis mechanism beta-poly (L-malic acid) Glucose-6-phosphate dehydrogenase Phosphoenolpyruvate carboxylase
ISSN号	1618-0240
DOI	10.1002/elsc.201700209
英文摘要	beta-poly (L-malic acid) (PMLA) is a biopolyester which has attracted industrial interest for its potential application in medicine and other industries. A high dissolved oxygen concentration (DO) was beneficial for PMLA production, while the mechanisms of DO in PMLA biosynthesis by Aureobasidium pullulans are still poorly understood. In this work, the amount of PMLA was first compared when A. pullulans ipe-1 were cultured under a high DO level (70% saturation) and a low DO level (10% saturation). Meanwhile, the key enzymes involved in different pathways of the precursor L-malic acid biosynthesis were studied. The results revealed that the activities of glucose-6-phosphate dehydrogenase (G6PDH) and phosphoenolpyruvate carboxylase (PEPC) were positively correlated with cell growth and PMLA production, while the activities of phosphofructokinases (PFK), pyruvic carboxylase (PC) and citrate synthetase (CS) did no show such correlations. It indicated that the Pentose Phosphate Pathway (PPP) may play a vital role in cell growth and PMLA biosynthesis. Moreover, the precursor L-malic acid for PMLA biosynthesis was mainly biosynthesized through phosphoenolpyruvic acid (PEP) via oxaloacetate catalyzed by PEPC. It was also found that low concentration of sodium fluoride (NaF) might impel carbon flux flow to the oxaloacetate through PEP, but inhibit the flux to the oxaloacetate via pyruvic acid.
资助项目	Beijing Natural Science Foundation[5182025] ; National Natural Science Foundation of China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005]
WOS关键词	DISSOLVED-OXYGEN CONCENTRATION ; PHYSARUM-POLYCEPHALUM ; CONTROL STRATEGY ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; SACCHAROMYCES-CEREVISIAE ; ENHANCED PRODUCTION ; ESCHERICHIA-COLI ; POLY(MALIC ACID) ; BATCH CULTURE ; BETA-POLY(L-MALATE)
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000434352900005
资助机构	Beijing Natural Science Foundation ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/24710]
专题	中国科学院过程工程研究所
通讯作者	Wan, Yinhua
作者单位	1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Qilu Univ Technol, Coll Food Sci & Engn, Jinan, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China 4.Tianjin Univ Sci & Technol, Coll Bioengn, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Yu, Haifeng,Liu, Bin,Luo, Jianquan,et al. Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1[J]. ENGINEERING IN LIFE SCIENCES,2018,18(6):379-386.
APA	Yu, Haifeng,Liu, Bin,Luo, Jianquan,Cao, Weifeng,Qiao, Changsheng,&Wan, Yinhua.(2018).Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1.ENGINEERING IN LIFE SCIENCES,18(6),379-386.
MLA	Yu, Haifeng,et al."Toward understanding the key enzymes involved in beta-poly (L-malic acid) biosynthesis by Aureobasidium pullulans ipe-1".ENGINEERING IN LIFE SCIENCES 18.6(2018):379-386.