Structural Insight into the Stabilizing Effect of O-Glycosylation

doi:10.1021/acs.biochem.7b00195

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	Structural Insight into the Stabilizing Effect of O-Glycosylation
	Chaffey, Patrick K.1,2; Guan, Xiaoyang 1,2; Chen, Chao 3,4; Ruan, Yuan 1,2; Wang, Xinfeng 1,2; Tran, Amy H.1,2; Koelsch, Theo N.1,2; Cui, Qiu 3,4; Feng, Yingang 3,4; Tan, Zhongping 1,2
刊名	BIOCHEMISTRY
	2017-06-13
卷号	56 期号:23 页码:2897-2906
DOI	10.1021/acs.biochem.7b00195
文献子类	Article
英文摘要	Protein glycosylation has been shown to have a variety of site specific and glycan-specific effects, but so far, the molecular logic that lead's to such observations has been elusive. Understanding the structural changes that Occur and being able to correlate those with the physical properties of the glycopeptide are valuable steps toward being able to predict how specific glycosylation patterns will affect the stability of glycoproteins: By systematically comparing the structural features of the O-glycosylated carbohydrate-binding module of a Trichoderma reesei-derived Family 7 cellobiohydrolase, we were able to develop a better understanding of the influence of O-glycan structure on the molecule's physical stability. Our results indicate that the previously observed stabilizing effects of O-glycans come from the introduction of new bonding interactions to the structure and increased rigidity, while the decreased stability seemed to result from the impaired interactions and increased conformational flexibility. This type of knowledge provides a powerful and potentially general mechanism for improving the stability of proteins through glycoengineering.
WOS关键词	CARBOHYDRATE-BINDING MODULE ; N-LINKED GLYCANS ; CHEMOENZYMATIC SYNTHESIS ; CHEMICAL GLYCOBIOLOGY ; PROTEIN STRUCTURES ; BIOLOGICAL ROLES ; NMR ANALYSIS ; DYNAMICS ; GLYCOPEPTIDE ; RELAXATION
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
WOS记录号	WOS:000403529900005
资助机构	University of Colorado Boulder ; National Science Foundation CAREER Award(CHE-1454925) ; National Natural Science Foundation of China(31670735 ; Chinese Government Scholarship(201604910035) ; 31661143023)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9708]
专题	青岛生物能源与过程研究所_代谢物组学团队
作者单位	1.Univ Colorado, Dept Chem & Biochem, Boulder, CO 80303 USA 2.Univ Colorado, BioFrontiers Inst, Boulder, CO 80303 USA 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chaffey, Patrick K.,Guan, Xiaoyang,Chen, Chao,et al. Structural Insight into the Stabilizing Effect of O-Glycosylation[J]. BIOCHEMISTRY,2017,56(23):2897-2906.
APA	Chaffey, Patrick K..,Guan, Xiaoyang.,Chen, Chao.,Ruan, Yuan.,Wang, Xinfeng.,...&Tan, Zhongping.(2017).Structural Insight into the Stabilizing Effect of O-Glycosylation.BIOCHEMISTRY,56(23),2897-2906.
MLA	Chaffey, Patrick K.,et al."Structural Insight into the Stabilizing Effect of O-Glycosylation".BIOCHEMISTRY 56.23(2017):2897-2906.