The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803

doi:10.3389/fmicb.2017.01176

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 微生物代谢工程团队

	The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803
	Song, Kuo 1; Hagemann, Martin 2; Tan, Xiaoming 1; Lu, Xuefeng 1,3
刊名	FRONTIERS IN MICROBIOLOGY
	2017-06-26
卷号	8
关键词	Slr1588 Synechocystis Spsa Glucosylglycerol Sucrose Ggpp Response Regulator Transcriptional Regulation
DOI	10.3389/fmicb.2017.01176
文献子类	Article
英文摘要	Cyanobacterial sucrose biosynthesis is stimulated under salt stress, which could be used for biotechnological sugar production. It has been shown that the response regulator Slr1588 negatively regulates the spsA gene encoding sucrose-phosphate synthase and mutation of the slr1588 gene also affected the salt tolerance of Synechocystis (Chen et al., 2014). The latter finding is contrary to earlier observations (Hagemann et al., 1997b). Here, we observed that ectopic expression of slr1588 did not restore the salt tolerance of the slr1588 mutant, making the essential function of this response regulator for salt tolerance questionable. Subsequent experiments showed that deletion of the entire coding sequence of slr1588 compromised the expression of the downstream situated ggpP gene, which encodes glucosylglycerol-phosphate phosphatase for synthesis of the primary osmolyte glucosylglycerol. Mutation of Delta slr1588 by deleting the N-terminal part of this protein (Delta slr1588-F976) did not affect ggpP expression, glucosylglycerol accumulation as well as salt tolerance, while the mutation of ggpP resulted in the previously reported salt-sensitive phenotype. In the Delta slr1588-F976 mutant spsA was up-regulated but sucrose content was lowered due to increased invertase activity. Our results reveal that Slr1588 is acting as a repressor for spsA as previously suggested but it is not crucial for the overall salt acclimation of Synechocystis.
WOS关键词	SP PCC-6803 ; PHOSPHATE-SYNTHASE ; COMPATIBLE SOLUTE ; CYANOBACTERIA ; GENE ; SUCROSE ; GLUCOSYLGLYCEROL ; EXPRESSION ; ENCODES ; ACCUMULATION
WOS研究方向	Microbiology
语种	英语
WOS记录号	WOS:000404006100002
资助机构	National Science Fund for Distinguished Young Scholars of China(31525002) ; Joint Sino-German Research Project(GZ 984) ; Shandong Taishan Scholarship ; National Science Foundation of China(31301018) ; Key Research Program of the Chinese Academy of Sciences(ZDRW-ZS-2016-3)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9644]
专题	青岛生物能源与过程研究所_微生物代谢工程团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Univ Rostock, Inst Biol Sci, Dept Plant Physiol, Rostock, Germany 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Song, Kuo,Hagemann, Martin,Tan, Xiaoming,et al. The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803[J]. FRONTIERS IN MICROBIOLOGY,2017,8.
APA	Song, Kuo,Hagemann, Martin,Tan, Xiaoming,&Lu, Xuefeng.(2017).The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803.FRONTIERS IN MICROBIOLOGY,8.
MLA	Song, Kuo,et al."The Response Regulator Slr1588 Regulates spsA But Is Not Crucial for Salt Acclimation of Synechocystis sp PCC 6803".FRONTIERS IN MICROBIOLOGY 8(2017).