Description of a Zostera marina catalase gene involved in responses to temperature stress

doi:10.7717/peerj.4532

	Description of a Zostera marina catalase gene involved in responses to temperature stress
	Zang, Yu 1; Liu, Jiao 3; Tang, Xue Xi 1,2; Zhou, Bin 1,2
刊名	PEERJ
	2018-03-26
卷号	6
关键词	Cat Temperature Stress Mrna Expression Enzyme Activity
ISSN号	2167-8359
DOI	10.7717/peerj.4532
文献子类	Article
英文摘要	Catalase (CAT) is an antioxidant enzyme that plays a significant role in cellular protection against oxidative darnage by degradation of hydrogen peroxide to oxygen and water. In the present study, the complete CAT cDNA sequence of Zostera marina was identified through expressed sequence tags (EST) analysis and the rapid amplification of cDNA ends (RACE) technique. The nucleotide sequence of ZmCAT cDNA consisted of 1,816 by with a 1,434 bp open reading frame (ORF) encoding a polypeptide of 477 amino acid residues, which possessed significant homology to other known plant CATs. The molecular mass of the predicted protein was 55.3 kDa with an estimated isoelectric point of 6.40. Phylogenetic analysis showed that ZmCAT was closely related to CAT from gramineous species. In response to temperature stress, H2O2 and MDA contents in Z. marina-increased significantly with cold stress (<10 degrees C) and heat stress (>25 degrees C). ZmCAT expression was significantly upregulated at temperatures from 5 to 10 degrees C and then gradually downregulated, reaching its lowest expression at 30 degrees C. Recombinant ZmCAT protein exhibited strong antioxidant activity over a wide temperature range, with the highest rZmCAT activity observed at 25 degrees C and a higher relative activity retained even with heat stress. All these results indicated that ZmCAT was a member of the plant CAT family and involved in minimizing oxidative damage effects in Z. marina under temperature stress.
WOS关键词	OXIDATIVE STRESS ; HEAT-STRESS ; SEAGRASSES ; TOLERANCE ; EXPRESSION ; L. ; MECHANISMS ; ENZYMES ; GROWTH ; USA
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	PEERJ INC
WOS记录号	WOS:000428696100002
资助机构	National Natural Science Foundation of China(41476091) ; Key Special Program on "Marine Environmental Security Protection'' of National Research and Development Project of China(2016YFC1402305)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10817]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhou, Bin
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zang, Yu,Liu, Jiao,Tang, Xue Xi,et al. Description of a Zostera marina catalase gene involved in responses to temperature stress[J]. PEERJ,2018,6.
APA	Zang, Yu,Liu, Jiao,Tang, Xue Xi,&Zhou, Bin.(2018).Description of a Zostera marina catalase gene involved in responses to temperature stress.PEERJ,6.
MLA	Zang, Yu,et al."Description of a Zostera marina catalase gene involved in responses to temperature stress".PEERJ 6(2018).