Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum
Liu, Ya-Jun1; Liu, Shiyue1,2; Dong, Sheng1; Li, Renmin1,2; Feng, Yingang1; Cui, Qiu1
刊名BIOTECHNOLOGY FOR BIOFUELS
2018-01-13
卷号11
关键词Activity Cellulosome Crystalline Cellulose Exocellulase Lignocellulose Substrate Specificity
ISSN号1754-6834
DOI10.1186/s13068-017-1009-4
文献子类Article
英文摘要Background: Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles and influences the activity and features of cellulosome to a great extent. Thus, it is of great importance to reveal the enzymatic features of Cel48S. However, Cel48S has not been well performed due to difficulties in purifying either recombinant or native Cel48S proteins.
WOS关键词MAJOR CELLULOSOME COMPONENT ; CRYSTALLINE CELLULOSE ; PHOSPHORIC-ACID ; IN-VITRO ; COMPLEX ; CELS ; HYDROLYSIS ; CELLOBIOHYDROLASE ; NANOMACHINES ; CLONING
WOS研究方向Biotechnology & Applied Microbiology ; Energy & Fuels
语种英语
出版者BIOMED CENTRAL LTD
WOS记录号WOS:000419918900002
资助机构National Key Technology Research and Development Program of China(2015BAD15B05) ; National Natural Science Foundation of China(31470210 ; Shandong Province Key Laboratory Union of Carbohydrate Industry Science and Technology(2015LKH102) ; 31570029 ; 31670735)
内容类型期刊论文
源URL[http://ir.qibebt.ac.cn/handle/337004/10549]  
专题青岛生物能源与过程研究所_代谢物组学团队
通讯作者Liu, Ya-Jun; Feng, Yingang; Cui, Qiu
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, CAS Key Lab Biofuels,Shandong Prov Key Lab Energy, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Liu, Ya-Jun,Liu, Shiyue,Dong, Sheng,et al. Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11.
APA Liu, Ya-Jun,Liu, Shiyue,Dong, Sheng,Li, Renmin,Feng, Yingang,&Cui, Qiu.(2018).Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum.BIOTECHNOLOGY FOR BIOFUELS,11.
MLA Liu, Ya-Jun,et al."Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum".BIOTECHNOLOGY FOR BIOFUELS 11(2018).
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