Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection

doi:10.1016/j.dci.2017.10.008

	Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection
	Li, Mo-Fei 1,2; Sun, Li 1,2
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2018-02-01
卷号	79 页码:142-149
关键词	Complement Regulatory Protein Complement Activation Cynoglossus Semilaevis Bacterial Infection
ISSN号	0145-305X
DOI	10.1016/j.dci.2017.10.008
文献子类	Article
英文摘要	In mammals, membrane-associated complement regulatory proteins (MCRP) can protect host cells from the damaging of the activated complement. In teleost, few studies on the function of MCRP have been documented. In the present report, we identified a MCRP (named CsMCRP) from the teleost fish tongue sole Cynoglossus semilaevis and examined its immune function. CsMCRP shares moderate sequence identities with fish DAF-like molecules. CsMCRP was predicted to be a transmembrane protein with three short consensus repeats located in the extracellular region. CsMCRP expression occurred in nine different tissues, especially blood, and in peripheral blood leukocytes (PBL). Recombinant CsMCRP inhibited complement activation and interacted with bacterial pathogen, the latter in a highly selective manner. Antibody blocking the CsMCRP on PBL significantly inhibited bacterial infection of PBL. These results indicate that teleost CsMCRP is both a regulator of complement activation and a cellular receptor involved in bacterial invasion. (C) 2017 Elsevier Ltd. All rights reserved.
WOS关键词	DECAY-ACCELERATING FACTOR ; SOLE CYNOGLOSSUS-SEMILAEVIS ; FACTOR DAF ; ALTERNATIVE PATHWAY ; COFACTOR PROTEIN ; FACTOR CD55 ; IN-VIVO ; CELLS ; EXPRESSION ; SYSTEM
WOS研究方向	Fisheries ; Immunology ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000422811500016
资助机构	National Natural Science Foundation of China(31330081) ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Taishan Scholar Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10547]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Mo-Fei,Sun, Li. Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,79:142-149.
APA	Li, Mo-Fei,&Sun, Li.(2018).Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,79,142-149.
MLA	Li, Mo-Fei,et al."Characterization of a teleost membrane-associated protein that is involved in the regulation of complement activation and bacterial infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 79(2018):142-149.