Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season

doi:10.17221/89/2017-CJAS

	Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season
	Shaliutina-Kolesova, Anna 1,2; Rui, Nian 1; Ashtiani, Saeed 1; Rodina, Marek 2; Cosson, Jacky 2; Linhart, Otomar 2
刊名	CZECH JOURNAL OF ANIMAL SCIENCE
	2018
卷号	63 期号:2 页码:78-84
关键词	Reproductive Season Lipid Peroxidation Products Fish Sperm Superoxide Dismutase Glutathione Reductase Glutathione Peroxidase
ISSN号	1212-1819
DOI	10.17221/89/2017-CJAS
文献子类	Article
英文摘要	Assessment of seminal oxidative stress indicators is emerging as a valuable prognostic tool in assisted reproductive technology. We investigated levels of oxidative stress caused by reactive oxygen species (ROS) and the antioxidant enzyme defence system comprising superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GPx) in seminal plasma (SP) of common carp Cyprinus carpio and rainbow trout Oncorhynchus mykiss over the course of the spawning season. Oxidation was determined in lipids and proteins by assessing thiobarbituric acid reactive species (TBARS) and the 2,4-dinitrophenylhydrazine carbonyl groups, respectively. Levels of SOD were assessed by the autoxidation of pyrogallol. Determination of GR and GPx was based on the rate of NADPH oxidation. We observed clear alterations in lipids and proteins over the course of the spawning season in both species. The highest levels of TBARS and CP were recorded late in the season. SOD was not significantly altered in either species. The activity of GR was higher in carp SP late in spawning compared to other times (P < 0.05), while in rainbow trout, GR significantly increased (24.13 +/- 2.8 mU/mg protein) in mid-season. A significantly lower GPx activity (9.18 +/- 1.32 mU/mg protein) was found in rainbow trout SP early in the spawning season, but no significant differences in GPx were observed over the course of the season in carp. These results provide further understanding of the role of fish SP antioxidants and present new data on the oxidant and antioxidant balance in SP during the spawning season that may be of value in the development of methods for artificial reproduction of teleost species.
WOS关键词	DNA INTEGRITY ; SPERM ; CRYOPRESERVATION ; GLUTATHIONE ; SEMEN ; PARAMETERS ; MOTILITY ; SPERMATOZOA ; INFERTILITY ; FERTILITY
WOS研究方向	Agriculture
语种	英语
出版者	CZECH ACADEMY AGRICULTURAL SCIENCES
WOS记录号	WOS:000422902200005
资助机构	Ministry of Education, Youth and Sports of the Czech Republic(CZ.1.05/2.1.00/01.0024 ; Food and Agriculture COST Action FA1205: AQUAGAMETE ; CAS President's International Fellowship for Postdoctoral Researchers(2017PB0060) ; National Natural Science Foundation of China(21676286) ; Primary Research Development Plan of Shandong Province(2016GSF121006) ; LO1205)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10541]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Shaliutina-Kolesova, Anna
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Vodnany, Czech Republic
推荐引用方式 GB/T 7714	Shaliutina-Kolesova, Anna,Rui, Nian,Ashtiani, Saeed,et al. Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season[J]. CZECH JOURNAL OF ANIMAL SCIENCE,2018,63(2):78-84.
APA	Shaliutina-Kolesova, Anna,Rui, Nian,Ashtiani, Saeed,Rodina, Marek,Cosson, Jacky,&Linhart, Otomar.(2018).Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season.CZECH JOURNAL OF ANIMAL SCIENCE,63(2),78-84.
MLA	Shaliutina-Kolesova, Anna,et al."Oxidative Stress and Antioxidant Enzyme Defence System in Seminal Plasma of Common Carp (Cyprinus carpio) and Rainbow Trout (Oncorhynchus mykiss) during Spawning Season".CZECH JOURNAL OF ANIMAL SCIENCE 63.2(2018):78-84.