Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)

doi:10.1534/g3.117.300210

	Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)
	Song, Hao 1,2,3; Qi, Lu 4; Zhang, Tao 1,2; Wang, Hai-yan 1,2
刊名	G3-GENES GENOMES GENETICS
	2017-12-01
卷号	7 期号:12 页码:3999-4008
关键词	Mirna Metamorphic Transition Gastropod Larval
ISSN号	2160-1836
DOI	10.1534/g3.117.300210
文献子类	Article
英文摘要	The veined rapa whelk (Rapana venosa) is widely consumed in China. Nevertheless, it preys on oceanic bivalves, thereby reducing this resource worldwide. Its larval metamorphosis comprises a transition from pelagic to benthic form, which involves considerable physiological and structural changes and has vital roles in its natural populations and commercial breeding. Thus, understanding the endogenous microRNAs (miRNAs) that drive metamorphosis is of great interest. This is the first study to use high-throughput sequencing to examine the alterations in miRNA expression that occur during metamorphosis in a marine gastropod. A total of 195 differentially expressed miRNAs were obtained. Sixty-five of these were expressed during the transition from precompetent to competent larvae. Thirty-three of these were upregulated and the others were downregulated. Another 123 miRNAs were expressed during the transition from competent to postlarvae. Ninety-six of these were upregulated and the remaining 27 were downregulated. The expression ofmiR-276-y, miR-100-x, miR-183-x, and miR-263-x showed a >100-fold change during development, while the miR-242-x and novel-m0052-3p expression levels changed over 3000-fold. Putative target gene coexpression, gene ontology, and pathway analyses suggest that these miRNAs play important parts in cell proliferation, migration, apoptosis, metabolic regulation, and energy absorption. Twenty miRNAs and their target genes involved in ingestion, digestion, cytoskeleton, cell adhesion, and apoptosis were identified. Nine of them were analyzed with real-time polymerase chain reaction (PCR), which showed an inverse correlation between the miRNAs and their relative expression levels. Our data elucidate the role of miRNAs in R. venosa metamorphic transition and serve as a solid basis for further investigations into regulatory mechanisms of gastropod metamorphosis.
WOS关键词	GENE-EXPRESSION ; DROSOPHILA ; LET-7 ; RNA ; VALENCIENNES ; COMPETENCE ; MURICIDAE ; MIR-125 ; ROLES ; SEA
WOS研究方向	Genetics & Heredity
语种	英语
出版者	GENETICS SOCIETY AMERICA
WOS记录号	WOS:000417073900016
资助机构	National Natural Science Foundation of China (NSFC)(31572636) ; earmarked fund for Modern Agro-industry Technology Research System(CARS-49) ; Primary Research & Development Plan of Shandong Province(2016ZDJS06A02) ; Industry Leading Talent Project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers(U1606404) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA11020703)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10485]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhang, Tao; Wang, Hai-yan
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ocean Univ China, Coll Fisheries, Qingdao 266001, Peoples R China
推荐引用方式 GB/T 7714	Song, Hao,Qi, Lu,Zhang, Tao,et al. Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)[J]. G3-GENES GENOMES GENETICS,2017,7(12):3999-4008.
APA	Song, Hao,Qi, Lu,Zhang, Tao,&Wang, Hai-yan.(2017).Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa).G3-GENES GENOMES GENETICS,7(12),3999-4008.
MLA	Song, Hao,et al."Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)".G3-GENES GENOMES GENETICS 7.12(2017):3999-4008.