Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers

doi:10.1007/s12104-018-9832-5

	Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers
	Xie, Huayong 1,2; Zhao, Yongxiang 1,2; Wang, Jing 1,2; Zhang, Zhengfeng 2; Yang, Jun 2
刊名	BIOMOLECULAR NMR ASSIGNMENTS
	2018-10-01
卷号	12 期号:2 页码:323-328
关键词	Aquaporin Z Solid-state NMR Resonance assignment Reverse labeling Secondary structure
ISSN号	1874-2718
DOI	10.1007/s12104-018-9832-5
英文摘要	Aquaporin Z is the first identified prokaryotic water channel in Escherichia coli with a high water permeability and strict substrate selectivity. Here we report nearly complete (94% of amino acid residues) C-13 and N-15 chemical shift assignments of AqpZ reconstituted in the lipid bilayers using a set of 2D and 3D magic angle spinning solid-state NMR spectra. Secondary structure of AqpZ predicted from chemical shift assignments is generally similar to that of X-ray structure with a number of differences in loop and near-loop regions. The BMRB accession number of the assignments is 27244.
资助项目	National Key R&D Program of China[2016YFA0501200] ; National Key R&D Program of China[2017YFA0505400] ; National Natural Science Foundation of China[31770798] ; National Natural Science Foundation of China[21425523] ; National Natural Science Foundation of China[21405171] ; National Natural Science Foundation of China[21775161]
WOS关键词	PROTEIN SECONDARY STRUCTURE ; X-RAY-STRUCTURE ; ESCHERICHIA-COLI ; WATER PERMEATION ; CHANNEL ; AQPZ ; SELECTIVITY
WOS研究方向	Biophysics ; Spectroscopy
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000443824500020
资助机构	National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13088]
专题	中国科学院武汉物理与数学研究所
通讯作者	Yang, Jun
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Xie, Huayong,Zhao, Yongxiang,Wang, Jing,et al. Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers[J]. BIOMOLECULAR NMR ASSIGNMENTS,2018,12(2):323-328.
APA	Xie, Huayong,Zhao, Yongxiang,Wang, Jing,Zhang, Zhengfeng,&Yang, Jun.(2018).Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers.BIOMOLECULAR NMR ASSIGNMENTS,12(2),323-328.
MLA	Xie, Huayong,et al."Solid-state NMR chemical shift assignments of aquaporin Z in lipid bilayers".BIOMOLECULAR NMR ASSIGNMENTS 12.2(2018):323-328.