Calcium accelerates SNARE-mediated lipid mixing through modulating alpha-synuclein membrane interaction

doi:10.1016/j.bbamem.2018.03.025

	Calcium accelerates SNARE-mediated lipid mixing through modulating alpha-synuclein membrane interaction
	Zhang, Zeting 2; Jiang, Xin 1,2; Xu, Danrui 1,2; Zheng, Wenwen 1,2; Liu, Maili 2; Li, Conggang 2
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
	2018-09-01
卷号	1860 期号:9 页码:1848-1853
关键词	Alpha-synuclein Ca2++ Nmr Spectroscopy Membrane Binding Snare-mediated Membrane Fusion
ISSN号	0005-2736
DOI	10.1016/j.bbamem.2018.03.025
文献子类	Article
英文摘要	alpha-Synuclein is involved in Parkinson's disease, and its interaction with cell membrane is vital to its pathological and physiological functions. We have shown that Ca2+ can regulate alpha-synuclein membrane interaction, but the physiological role of Ca2+ in modulating alpha-synuclein membrane interaction is still unexplored. Based on the previous findings that alpha-synuclein inhibits membrane fusion and its inhibitory effect is highly related to its membrane binding, here we employed solution state Nuclear Magnetic Resonance (NMR) spectroscopy and the ensemble fluorescence fusion assay to show that Ca2+ can modulate the inhibitory effect of alpha-synuclein on SNARE-mediated membrane fusion through disrupting alpha-synuclein membrane interaction, resulting in acceleration of SNARE-mediated membrane fusion. These results suggest a modulatory effect of Ca2+ on membrane mediated normal function of alpha-synuclein, which of importance for the study of the Parkinson's disease.
WOS关键词	SOLUTION NMR-SPECTROSCOPY ; NEUROTRANSMITTER RELEASE ; VESICLE DOCKING ; BINDING ; PROTEIN ; FUSION ; AGGREGATION ; EXOCYTOSIS
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000442333600025
资助机构	National Natural Science Foundation of China(21203243 ; National Natural Science Foundation of China(21203243 ; 21673284) ; 21673284) ; National Natural Science Foundation of China(21203243 ; National Natural Science Foundation of China(21203243 ; 21673284) ; 21673284) ; National Natural Science Foundation of China(21203243 ; National Natural Science Foundation of China(21203243 ; 21673284) ; 21673284) ; National Natural Science Foundation of China(21203243 ; National Natural Science Foundation of China(21203243 ; 21673284) ; 21673284)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/13056]
专题	中国科学院武汉物理与数学研究所
通讯作者	Li, Conggang
作者单位	1.Chinese Acad Sci, Grad Univ, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Zeting,Jiang, Xin,Xu, Danrui,et al. Calcium accelerates SNARE-mediated lipid mixing through modulating alpha-synuclein membrane interaction[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,2018,1860(9):1848-1853.
APA	Zhang, Zeting,Jiang, Xin,Xu, Danrui,Zheng, Wenwen,Liu, Maili,&Li, Conggang.(2018).Calcium accelerates SNARE-mediated lipid mixing through modulating alpha-synuclein membrane interaction.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1860(9),1848-1853.
MLA	Zhang, Zeting,et al."Calcium accelerates SNARE-mediated lipid mixing through modulating alpha-synuclein membrane interaction".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1860.9(2018):1848-1853.