Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis

doi:10.1021/acs.analchem.7b05355

	Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis
	Yuan, Bi-Feng 2; Zhu, Quan-Fei 2; Guo, Ning 2; Zheng, Shu-Jian 2; Wang, Ya-Lan 2; Wang, Jie 1,3; Xu, Jing 2; Liu, Shi-Jie 2; He, Ke 2; Hu, Ting 1,3
刊名	ANALYTICAL CHEMISTRY
	2018-03-06
卷号	90 期号:5 页码:3512-3520
ISSN号	0003-2700
DOI	10.1021/acs.analchem.7b05355
文献子类	Article
英文摘要	Gut microbiota plays important roles in the host health. The host and symbiotic gut microbiota coproduce a large number of metabolites during the metabolism of food and xenobiotics. The analysis of fecal metabolites can provide a noninvasive manner to study the outcome of the host-gut microbiota interaction. Herein, we reported the comprehensive profiling of fecal metabolome of mice by an integrated chemical isotope labeling combined with liquid chromatography mass spectrometry (CIL-LC-MS) analysis. The metabolites are categorized into several submetabolomes based on the functional moieties (i.e., carboxyl, carbonyl, amine, and thiol) and then analysis of the individual submetabolome was performed. The combined data from the submetabolome form the metabolome with relatively high coverage. To this end, we synthesized stable isotope labeling reagents to label metabolites with different groups, including carboxyl, carbonyl, amine, and thiol groups. We detected 2302 potential metabolites, among which, 1388 could be positively or putatively identified in feces of mice. We then further confirmed 308 metabolites based on our established library of chemically labeled standards and tandem mass spectrometry analysis. With the identified metabolites in feces of mice, we established mice fecal metabolome database, which can be used to readily identify metabolites from feces of mice. Furthermore, we discovered 211 fecal metabolites exhibited significant difference between Alzheimer's disease (AD) model mice and wild type (WT) mice, which suggests the close correlation between the fecal metabolites and AD pathology and provides new potential biomarkers for the diagnosis of AD.
WOS关键词	LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY ; PRECURSOR ION SCAN ; GUT MICROBIOTA ; OXIDATION-PRODUCTS ; ALZHEIMERS-DISEASE ; BILE-ACID ; LC-MS ; DERIVATIZATION ; 5-METHYLCYTOSINE ; MAMMALS
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000427095700075
资助机构	National Key R&D Program of China(2017YFC0906800) ; National Key R&D Program of China(2017YFC0906800) ; National Natural Science Foundation of China(21635006 ; National Natural Science Foundation of China(21635006 ; 21475098 ; 21475098 ; 31670373 ; 31670373 ; 21522507 ; 21522507 ; 21672166) ; 21672166) ; National Key R&D Program of China(2017YFC0906800) ; National Key R&D Program of China(2017YFC0906800) ; National Natural Science Foundation of China(21635006 ; National Natural Science Foundation of China(21635006 ; 21475098 ; 21475098 ; 31670373 ; 31670373 ; 21522507 ; 21522507 ; 21672166) ; 21672166) ; National Key R&D Program of China(2017YFC0906800) ; National Key R&D Program of China(2017YFC0906800) ; National Natural Science Foundation of China(21635006 ; National Natural Science Foundation of China(21635006 ; 21475098 ; 21475098 ; 31670373 ; 31670373 ; 21522507 ; 21522507 ; 21672166) ; 21672166) ; National Key R&D Program of China(2017YFC0906800) ; National Key R&D Program of China(2017YFC0906800) ; National Natural Science Foundation of China(21635006 ; National Natural Science Foundation of China(21635006 ; 21475098 ; 21475098 ; 31670373 ; 31670373 ; 21522507 ; 21522507 ; 21672166) ; 21672166)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12906]
专题	中国科学院武汉物理与数学研究所
通讯作者	Feng, Yu-Qi
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Wuhan Univ, Dept Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Ctr Magnet Resonance, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 4.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Bi-Feng,Zhu, Quan-Fei,Guo, Ning,et al. Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis[J]. ANALYTICAL CHEMISTRY,2018,90(5):3512-3520.
APA	Yuan, Bi-Feng.,Zhu, Quan-Fei.,Guo, Ning.,Zheng, Shu-Jian.,Wang, Ya-Lan.,...&Feng, Yu-Qi.(2018).Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis.ANALYTICAL CHEMISTRY,90(5),3512-3520.
MLA	Yuan, Bi-Feng,et al."Comprehensive Profiling of Fecal Metabolome of Mice by Integrated Chemical Isotope Labeling-Mass Spectrometry Analysis".ANALYTICAL CHEMISTRY 90.5(2018):3512-3520.