Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats

doi:10.18632/oncotarget.22040

	Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats
	Zeng, Hao-Long 2; Yang, Qing 4; Du, Hongying 5; Li, Huijun 2; Shen, Ying 2; Liu, Taotao 2; Chen, Xi 4; Kamal, Ghulam Mustafa 6; Guan, Qing 2; Cheng, Liming 2
刊名	ONCOTARGET
	2017-11-24
卷号	8 期号:60 页码:102020-102032
关键词	Alcohol Preference Liver Mitochondria Quantitative Proteomics Metabolomics Tca Cycle
ISSN号	1949-2553
DOI	10.18632/oncotarget.22040
文献子类	Article
英文摘要	Alcohol preference induced tolerance in humans and animals when their bodily functions adapt to compensate for the disruption caused by alcohol consumption. This was thought to be an important component of the genetic predisposition to alcoholism. To investigate the underlying mechanisms of hepatic metabolic tolerance during alcohol preference, the alcohol preferring and alcohol non-preferring rats were used in this study. The liver mitochondria were purified for comparative quantitative proteomics analysis, and the liver metabolite extracts were collected for metabolomics analysis. Our study identified 96 differentially expressed hepatic mitochondrial proteins that associated with alcohol preference, the further gene ontology and protein interaction network analysis suggest a down-regulation of amino acid metabolism and up-regulation of lipid metabolism. We found alcohol preference induced a series of enzymes decreased (e.g. SSADH and GABA-T) and several amino acids increased (e.g. glutamate and aspartate) in rat liver, indicating down-regulations of glutamate degradation occurred during alcohol preference. Most of these changes were due to the genetic differences between alcohol preferring and non-preferring animals. Furthermore, this study would provided new insights to further clarify the mechanisms of hepatic metabolic tolerance during alcohol preference.
WOS关键词	LIVER-MITOCHONDRIA ; DRINKING ; DEPENDENCE ; ETHANOL ; EXPOSURE ; BRAIN ; GABA ; PHOSPHORYLATION ; SPECTROSCOPY ; CONSUMPTION
WOS研究方向	Oncology ; Cell Biology
语种	英语
出版者	IMPACT JOURNALS LLC
WOS记录号	WOS:000419533200085
资助机构	Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193) ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Ministry of Science and Technology(2015CB755601 ; Chinese Academy of Science(XDB02050005) ; Chinese Academy of Science(XDB02050005) ; National Natural Science Foundation of China(31600666 ; National Natural Science Foundation of China(31600666 ; 2015AA020508) ; 2015AA020508) ; 31771193) ; 31771193)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12834]
专题	中国科学院武汉物理与数学研究所
通讯作者	Wang, Jie; Xu, Fuqiang
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Hosp, Dept Lab Med, Tongji Med Coll, Wuhan, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance,Key Lab Magnet Resonan, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan, Hubei, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China 5.Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan, Hubei, Peoples R China 6.Univ Faisalabad, Dept Chem, Govt Coll, Faisalabad, Pakistan 7.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zeng, Hao-Long,Yang, Qing,Du, Hongying,et al. Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats[J]. ONCOTARGET,2017,8(60):102020-102032.
APA	Zeng, Hao-Long.,Yang, Qing.,Du, Hongying.,Li, Huijun.,Shen, Ying.,...&Xu, Fuqiang.(2017).Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats.ONCOTARGET,8(60),102020-102032.
MLA	Zeng, Hao-Long,et al."Proteomics and metabolomics analysis of hepatic mitochondrial metabolism in alcohol-preferring and non-preferring rats".ONCOTARGET 8.60(2017):102020-102032.