Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy

doi:10.1007/s00216-017-0745-4

	Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy
	Ye, Yansheng 2; Wu, Qiong 2; Zheng, Wenwen 2; Jiang, Bin 2; Pielak, Gary J.1,3; Liu, Maili 2; Li, Conggang 2
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2018
卷号	410 期号:3 页码:869-874
关键词	Proteins Rotational Motion Nmr Viscosity Weak Interactions
ISSN号	1618-2642
DOI	10.1007/s00216-017-0745-4
文献子类	Article
英文摘要	Protein diffusion in living cells might differ significantly from that measured in vitro. Little is known about the effect of globular protein size on rotational diffusion in cells because each protein has distinct surface properties, which result in different interactions with cellular components. To overcome this problem, the B1 domain of protein G (GB1) and several concatemers of the protein were labeled with 5-fluorotryptophan and studied by F-19 NMR in Escherichia coli cells, Xenopus laevis oocytes, and in aqueous solutions crowded with glycerol, or Ficoll70 (TM) and lysozyme. Relaxation data show that the size dependence of protein rotation in cells is due to weak interactions of the target protein with cellular components, but the effect of these interactions decreases as protein size increases. The results provide valuable information for interpreting protein diffusion data acquired in living cells.
WOS关键词	MAGNETIC-RESONANCE-SPECTROSCOPY ; XENOPUS-LAEVIS OOCYTES ; LIVING CELLS ; ESCHERICHIA-COLI ; MULTIDIMENSIONAL NMR ; QUINARY STRUCTURE ; IN-VIVO ; DIFFUSION ; CYTOPLASM ; ENVIRONMENTS
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000422952600017
资助机构	Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854) ; Ministry of Science and Technology of China(2017YFA0505400) ; Ministry of Science and Technology of China(2017YFA0505400) ; Inovation team of Hubei Province grant(2016CFA002) ; Inovation team of Hubei Province grant(2016CFA002) ; National Natural Sciences Foundation of China(21575156) ; National Natural Sciences Foundation of China(21575156) ; US National Science Foundation(MCB-1410854) ; US National Science Foundation(MCB-1410854)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12818]
专题	中国科学院武汉物理与数学研究所
通讯作者	Liu, Maili; Li, Conggang
作者单位	1.Univ North Carolina Chapel Hill, Lineberger Comprehens Canc Ctr, Chapel Hill, NC 27599 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Natl Ctr Magnet Resonance Wuhan, Key Lab Magnet Resonance Biol Syst,State Key Lab, Wuhan 430071, Hubei, Peoples R China 3.Univ North Carolina Chapel Hill, Dept Chem Biochem & Biophys, Chapel Hill, NC 27599 USA
推荐引用方式 GB/T 7714	Ye, Yansheng,Wu, Qiong,Zheng, Wenwen,et al. Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(3):869-874.
APA	Ye, Yansheng.,Wu, Qiong.,Zheng, Wenwen.,Jiang, Bin.,Pielak, Gary J..,...&Li, Conggang.(2018).Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(3),869-874.
MLA	Ye, Yansheng,et al."Quantification of size effect on protein rotational mobility in cells by F-19 NMR spectroscopy".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.3(2018):869-874.