Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP

doi:10.1038/s41598-017-16723-5

	Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP
	Hou, Meng-Meng 5,6; Polykretis, Panagis 4; Luchinat, Enrico 3,4; Wang, Xiao 5,6; Chen, Shen-Na 5,6; Zuo, Hui-Hui 5,6; Yang, Yin 5,6; Chen, Jia-Liang 5,6; Ye, Yansheng 1; Li, Conggang 1
刊名	SCIENTIFIC REPORTS
	2017-11-30
卷号	7
ISSN号	2045-2322
DOI	10.1038/s41598-017-16723-5
文献子类	Article
英文摘要	The X-chromosome linked inhibitor of apoptosis (XIAP) is a multidomain metalloprotein involved in caspase inhibition and in copper homeostasis. It contains three zinc-binding baculoviral IAP repeats (BIR) domains, which are responsible for caspase interaction. Recently, it has been suggested that the BIR domains can bind copper, however high resolution data on such interaction is missing. Here we characterize by NMR the structural properties of BIR1 in solution, and the effects of its interaction with copper both in vitro and in physiological environments. BIR1 is dimeric in solution, consistent with the X-ray structure. Cysteine 12, located in the unfolded N-terminal region, has a remarkably low redox potential, and is prone to oxidation even in reducing physiological environments. Interaction of BIR1 with copper(II) results in the oxidation of cysteine 12, with the formation of either an intermolecular disulfide bond between two BIR1 molecules or a mixed disulfide bond with glutathione, whereas the zinc binding site is not affected by the interaction.
WOS关键词	PARAMAGNETIC NMR-SPECTROSCOPY ; PROTEIN-PRODUCTION ; MAMMALIAN-CELLS ; INHIBITION ; BINDING ; APOPTOSIS ; OOCYTES
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000416891400032
资助机构	National Key R&D Program of China(2016YFA0501202) ; National Key R&D Program of China(2016YFA0501202) ; National Natural Science Foundation of China(21673122 ; National Natural Science Foundation of China(21673122 ; 21473095) ; 21473095) ; National Key R&D Program of China(2016YFA0501202) ; National Key R&D Program of China(2016YFA0501202) ; National Natural Science Foundation of China(21673122 ; National Natural Science Foundation of China(21673122 ; 21473095) ; 21473095) ; National Key R&D Program of China(2016YFA0501202) ; National Key R&D Program of China(2016YFA0501202) ; National Natural Science Foundation of China(21673122 ; National Natural Science Foundation of China(21673122 ; 21473095) ; 21473095) ; National Key R&D Program of China(2016YFA0501202) ; National Key R&D Program of China(2016YFA0501202) ; National Natural Science Foundation of China(21673122 ; National Natural Science Foundation of China(21673122 ; 21473095) ; 21473095)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12728]
专题	中国科学院武汉物理与数学研究所
通讯作者	Banci, Lucia; Su, Xun-Cheng
作者单位	1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China 2.Univ Florence, Dept Chem, I-50019 Florence, Italy 3.Univ Florence, Dept Biomed Clin & Expt Sci, I-50134 Florence, Italy 4.Univ Florence, Magnet Resonance Ctr CERM, I-50019 Florence, Italy 5.Nankai Univ, Res Inst Elementoorgan Chem, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Tianjin 300071, Peoples R China 6.Nankai Univ, State Key Lab, Tianjin 300071, Peoples R China
推荐引用方式 GB/T 7714	Hou, Meng-Meng,Polykretis, Panagis,Luchinat, Enrico,et al. Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP[J]. SCIENTIFIC REPORTS,2017,7.
APA	Hou, Meng-Meng.,Polykretis, Panagis.,Luchinat, Enrico.,Wang, Xiao.,Chen, Shen-Na.,...&Su, Xun-Cheng.(2017).Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP.SCIENTIFIC REPORTS,7.
MLA	Hou, Meng-Meng,et al."Solution structure and interaction with copper in vitro and in living cells of the first BIR domain of XIAP".SCIENTIFIC REPORTS 7(2017).