Analysis of drug-protein binding using nuclear magnetic resonance based molecular diffusion measurements

	Analysis of drug-protein binding using nuclear magnetic resonance based molecular diffusion measurements
	Liu, ML ; Nicholson, JK ; Lindon, JC
刊名	ANALYTICAL COMMUNICATIONS
	1997-08-01
卷号	34 期号:8 页码:225-228
ISSN号	1359-7337
文献子类	Article
英文摘要	H-1 and F-19 NMR spectroscopic approaches are presented for investigation of protein-ligand binding using the measurement of molecular diffusion coefficients, This has been exemplified by investigation of the binding of 4-trifluoromethylbenzoic acid (TFBA), and the non-steroidal anti-inflammatory drugs ibuprofen and flurbiprofen to human serum albumin (HSA). To avoid the need to define the number of independent binding sites and their individual dissociation constants, the relative binding of the ligands to HSA was quantified using a binding capacity, defined as log (ratio of ligand to HSA) required to give 50% of the ligand bound, The diffusion-based approach to the study of protein-ligand binding offers a number of advantages over previous NMR methods, which rely on the error-prone determination of bound-ligand chemical shifts, relaxation times or linewidths. The comparative simplicity of the diffusion method may allow its widespread use in the investigation of drug-macromolecule interactions, particularly for the weak but extensive binding of drugs to HSA in blood, which can affect biological activity in vivo.
WOS关键词	HUMAN SERUM-ALBUMIN ; GRADIENT
WOS研究方向	Chemistry
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:A1997XQ87400005
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/12713]
专题	中国科学院武汉物理与数学研究所
作者单位	1.UNIV LONDON BIRKBECK COLL,DEPT CHEM,LONDON WC1H 0PP,ENGLAND 2.CHINESE ACAD SCI,WUHAN INST PHYS & MATH,LAB MAGNET RESONANCE & ATOM & MOL PHYS,WUHAN 430071,PEOPLES R CHINA
推荐引用方式 GB/T 7714	Liu, ML,Nicholson, JK,Lindon, JC. Analysis of drug-protein binding using nuclear magnetic resonance based molecular diffusion measurements[J]. ANALYTICAL COMMUNICATIONS,1997,34(8):225-228.
APA	Liu, ML,Nicholson, JK,&Lindon, JC.(1997).Analysis of drug-protein binding using nuclear magnetic resonance based molecular diffusion measurements.ANALYTICAL COMMUNICATIONS,34(8),225-228.
MLA	Liu, ML,et al."Analysis of drug-protein binding using nuclear magnetic resonance based molecular diffusion measurements".ANALYTICAL COMMUNICATIONS 34.8(1997):225-228.