Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe

doi:10.1016/j.talanta.2018.03.039

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe
	Liu, Maili 1; Liu, Hongbin 1; Chen, Shizhen 1; Yang, Shengjun 1; Guo, Qianni 1; Zeng, Qingbin 1; Zhou, Xin 1; McMahon, Michael T.2,3
刊名	TALANTA
	2018-07-01
卷号	184 页码:513-519
关键词	F-19 Nuclear Magnetic Resonance Biothiols Probes Thiol-addition/intramolecular Cyclization
DOI	10.1016/j.talanta.2018.03.039
文献子类	Article
英文摘要	Simultaneous detection and differentiation of biomolecules is of significance in biological research. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) play an important role in regulating the vital functions of living organisms. However, existing methods for simultaneous detection and differentiation of Cys, Hcy, and GSH are still challenging because of their similarity in structure and chemical properties. Herein we report a probe that simultaneously detects and discriminates between mixtures of Cys, Hcy and GSH using F-19 nuclear magnetic resonance (NMR). This F-19 NMR probe responds rapidly to biothiols through the Michael addition reaction and subsequent intramolecular cyclization reaction allowing differentiation between Cys, Hcy and GSH through F-19 NMR chemical shift. We demonstrate that this F-19 NMR probe is a powerful method for analysis of complex mixtures.
WOS关键词	HIGHLY SELECTIVE DETECTION ; MASS-SPECTROMETRY ; FLUORESCENT-PROBE ; REACTIVE OXYGEN ; CONTRAST AGENTS ; LIVING CELLS ; IN-VIVO ; CYSTEINE ; GLUTATHIONE ; MRI
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:000432234900066
资助机构	National Natural Science Foundation of China(81625011 ; National Natural Science Foundation of China(81625011 ; National Key R&D Program of China(2016YFC1304700 ; National Key R&D Program of China(2016YFC1304700 ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; 81227902) ; 81227902) ; 2017YFA0505400) ; 2017YFA0505400) ; National Natural Science Foundation of China(81625011 ; National Natural Science Foundation of China(81625011 ; National Key R&D Program of China(2016YFC1304700 ; National Key R&D Program of China(2016YFC1304700 ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; Key Research Program of Frontier Sciences, CAS(QYZDY-SSW-SLH018) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; National Program for Support of Eminent Professionals (National Program for Support of Top-notch Young Professionals) ; 81227902) ; 81227902) ; 2017YFA0505400) ; 2017YFA0505400)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11984]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Chinese Acad Sci, Natl Ctr Magnet Resonance Wuhan, Wuhan Inst Phys & Math,Key Lab Magnet Resonance B, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China 2.Kennedy Krieger Inst, FM Kirby Res Ctr Funct Brain Imaging, Baltimore, MD 21205 USA 3.Johns Hopkins Sch Med, Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA
推荐引用方式 GB/T 7714	Liu, Maili,Liu, Hongbin,Chen, Shizhen,et al. Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe[J]. TALANTA,2018,184:513-519.
APA	Liu, Maili.,Liu, Hongbin.,Chen, Shizhen.,Yang, Shengjun.,Guo, Qianni.,...&McMahon, Michael T..(2018).Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe.TALANTA,184,513-519.
MLA	Liu, Maili,et al."Detection and differentiation of Cys, Hcy and GSH mixtures by F-19 NMR probe".TALANTA 184(2018):513-519.