Specific patterns of spinal metabolites underlying alpha-Me-5-HT-evoked pruritus compared with histamine and capsaicin assessed by proton nuclear magnetic resonance spectroscopy

doi:10.1016/j.bbadis.2017.03.011

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Specific patterns of spinal metabolites underlying alpha-Me-5-HT-evoked pruritus compared with histamine and capsaicin assessed by proton nuclear magnetic resonance spectroscopy
	Tian, Xuebi 1; Liu, Taotao 1,3; Liu, Zeyuan 5; Li, Zhixiao 1; Kamal, Ghulam Mustafa 2,4; He, Zhigang 1; Xiang, Hongbing 1; Wang, Jie 2,4; Xu, Fuqiang 2,4; Liu, Huili 2
刊名	BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
	2017-06-01
卷号	1863 期号:6 页码:1222-1230
关键词	Itch Spinal Cord Metabolites Nuclear Magnetic Resonance Spectroscopy Gaba
DOI	10.1016/j.bbadis.2017.03.011
文献子类	Article
英文摘要	The mechanism behind itching is not well understood. Proton nuclear magnetic resonance (H-1-NMR) spectroscopic analysis of spinal cord extracts provides a quick modality for evaluating the specific metabolic activity of alpha-Me-5-HT-evoked pruritus mice. In the current study, four groups of young adult male C5781/6 mice were investigated; one group treated with saline, while the other groups intradermally injected with alpha-Me-5-HT (histamine independent pruritogen), histamine (histamine dependent pruritogen) and capsaicin (algogenic substance), respectively. The intradermal microinjection of alpha-Me-5-HT and histamine resulted in a dramatic increase in the itch behavior. Furthermore, the results of NMR studies of the spinal cord extracts revealed that the metabolites show very different patterns for these different drugs, especially when comparing alpha-Me-5-HT and capsaicin. All the animals in the groups of alpha-Me-5-HT and capsaicin were completely separated using the metabolite parameters and principal component analysis. For alpha a-Me-5-HT, the concentrations of glutamate, GABA, glycine and aspartate increased significantly, especially for GABA (increased 17.2%, p = 0.008). Furthermore, the concentration of NAA increased, but there was no significant difference (increased 11.3%, p = 0.191) compared to capsaicin (decreased 29.1%, p = 0.002). Thus the application of magnetic resonance spectroscopy technique, coupled with statistical analysis, could further explain the mechanism behind itching evoked by alpha-Me-5-HT or other drugs. It can thus improve our understanding of itch pathophysiology and pharmacological therapies which may contribute to itch relief.
WOS关键词	CHRONIC ETHANOL EXPOSURE ; DORSAL-HORN ; RAT-BRAIN ; CEREBROSPINAL-FLUID ; RECEPTOR AGONISTS ; NMR-SPECTROSCOPY ; HIGH-RESOLUTION ; ITCH SENSATION ; CORD-INJURY ; PAIN
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种	英语
WOS记录号	WOS:000404704600007
资助机构	Chinese Ministry of Science and Technology(2015AA020508) ; Chinese Ministry of Science and Technology(2015AA020508) ; National Natural Science Foundation of China(81271766) ; National Natural Science Foundation of China(81271766) ; National Natural Science Foundation of Hubei Province(2013CFB121) ; National Natural Science Foundation of Hubei Province(2013CFB121) ; Chinese Ministry of Science and Technology(2015AA020508) ; Chinese Ministry of Science and Technology(2015AA020508) ; National Natural Science Foundation of China(81271766) ; National Natural Science Foundation of China(81271766) ; National Natural Science Foundation of Hubei Province(2013CFB121) ; National Natural Science Foundation of Hubei Province(2013CFB121)
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/11465]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Huazhong Univ Sci & Technol, Tongji Hosp, Dept Anesthesiol & Pain Med, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan 430071, Hubei, Peoples R China 3.Peking Univ, Hosp 3, Dept Anesthesiol, Beijing 100191, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Wuhan Univ, Coll Life Sci, Wuhan 430076, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Tian, Xuebi,Liu, Taotao,Liu, Zeyuan,et al. Specific patterns of spinal metabolites underlying alpha-Me-5-HT-evoked pruritus compared with histamine and capsaicin assessed by proton nuclear magnetic resonance spectroscopy[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2017,1863(6):1222-1230.
APA	Tian, Xuebi.,Liu, Taotao.,Liu, Zeyuan.,Li, Zhixiao.,Kamal, Ghulam Mustafa.,...&Liu, Huili.(2017).Specific patterns of spinal metabolites underlying alpha-Me-5-HT-evoked pruritus compared with histamine and capsaicin assessed by proton nuclear magnetic resonance spectroscopy.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,1863(6),1222-1230.
MLA	Tian, Xuebi,et al."Specific patterns of spinal metabolites underlying alpha-Me-5-HT-evoked pruritus compared with histamine and capsaicin assessed by proton nuclear magnetic resonance spectroscopy".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1863.6(2017):1222-1230.